Complementation between mutants of CHO cells resistant to a variety of plant lectins

Pamela Stanley, Louis Siminovitch

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Chinese hamster cell mutants resistant to the lectins PHA, WGA, RIC, LCA, and CON A were previously grouped into 8-10 distinct phenotypes on the basis of their unique patterns of lectin resistance and lectin-binding properties. All but one of these classes of lectin-resistant (LecR) mutants behave recessively in somatic cell hybrids. One ricin-resistant class ( {Mathematical expression} behaves dominantly. Test for complementation, by measuring the lectin-resistant properties of appropriate hybrids, show that seven distinct complementation groups can be delineated among the phenotypically recessive mutants.

Original languageEnglish (US)
Pages (from-to)391-405
Number of pages15
JournalSomatic Cell Genetics
Volume3
Issue number4
DOIs
StatePublished - Jul 1977
Externally publishedYes

Fingerprint

Plant Lectins
CHO Cells
Lectins
Genetic Complementation Test
Ricin
Hybrid Cells
Cricetulus
Phenotype

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Complementation between mutants of CHO cells resistant to a variety of plant lectins. / Stanley, Pamela; Siminovitch, Louis.

In: Somatic Cell Genetics, Vol. 3, No. 4, 07.1977, p. 391-405.

Research output: Contribution to journalArticle

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