Complementary approaches to investigating cancer cell dynamics in the tumor microenvironment

Michael R. Padgen, Waseem Khan Raja, Bojana Gligorijevic, James K. Williams, John S. Condeelis, James Castracane

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Metastatic cancer cells respond to chemical and mechanical stimuli in their microenvironment that guide invasion into the surrounding tissue and eventually the circulatory/lymph systems. The NANIVID is designed to be an in vivo device used to collect metastatic cancer cells by providing a gradient of epidermal growth factor through the controlled release from a customized hydrogel. The model cells, MTLn3 and MenaInv, both derived from a rat mammary adenocarcinoma, will migrate toward the device and be collected in the chamber. A set of electrodes inside the chamber will provide real-time data on the density of cells collected in the device. The characterization and optimization of the electrodes in vitro will be reported, as will the development of an equivalent circuit model used to describe electrode behavior. The ultimate goal of this work is for the NANIVID to be used for in vivo investigations of a rat model of mammary cancer. Furthermore, since the morphology, mechanical properties, and movement of cells are influenced by the microenvironment, a combined scanning confocal laser microscope and atomic force microscope will be used to study these relationships. This work will further the understanding of the dynamics and mechanics of metastatic cancer cells as they leave the primary tumor and metastasize.

Original languageEnglish (US)
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems IX
DOIs
StatePublished - Jun 9 2011
EventMicrofluidics, BioMEMS, and Medical Microsystems IX - San Francisco, CA, United States
Duration: Jan 23 2011Jan 25 2011

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7929
ISSN (Print)0277-786X

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems IX
CountryUnited States
CitySan Francisco, CA
Period1/23/111/25/11

Keywords

  • AFM
  • MTLn3
  • Mena
  • impedance spectroscopy
  • metastasis
  • tumor microenvironment

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Padgen, M. R., Raja, W. K., Gligorijevic, B., Williams, J. K., Condeelis, J. S., & Castracane, J. (2011). Complementary approaches to investigating cancer cell dynamics in the tumor microenvironment. In Microfluidics, BioMEMS, and Medical Microsystems IX [792905] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7929). https://doi.org/10.1117/12.873421