Colony stimulating factor-1 receptor signaling networks inhibit mouse macrophage inflammatory responses by induction of microRNA-21

Cristina I. Caescu, Xingyi Guo, Lydia Tesfa, Tushar D. Bhagat, Amit K. Verma, Deyou Zheng, E. Richard Stanley

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Macrophage polarization between the M2 (repair, protumorigenic) and M1 (inflammatory) phenotypes is seen as a continuum of states. The detailed transcriptional events and signals downstream of colony-stimulating factor 1 receptor (CSF-1R) that contributes to amplification of the M2 phenotype and suppression of the M1 phenotype are largely unknown. Macrophage CSF-1R pTyr-721 signaling promotes cell motility and enhancement of tumor cell invasion in vitro. Combining analysis of cellular systems for CSF-1Rgain of function and loss of functionwith bioinformatic analysis of themacrophage CSF-1R pTyr-721-regulated transcriptome, we uncovered microRNA-21 (miR-21) as a downstream molecular switch controlling macrophage activation and identified extracellular signal-regulated kinase1/2 and nuclear factor-kB as CSF-1R pTyr-721-regulated signaling nodes.Weshow that CSF-1R pTyr-721 signaling suppresses the inflammatory phenotype, predominantly by induction of miR-21. Profiling of the miR-21-regulated messenger RNAs revealed that 80% of the CSF-1-regulated canonical miR-21 targets are proinflammatory molecules. Additionally, miR-21 positively regulates M2 marker expression. Moreover, miR-21 feeds back to positively regulate its own expression and to limit CSF-1R-mediated activation of extracellular signal-regulated kinase1/2 and nuclear factor-kB. Consistent with an anti-inflammatory role of miRNA-21, intraperitoneal injection of mice with a miRNA-21 inhibitor increases the recruitment of inflammatory monocytes and enhances the peritoneal monocyte/macrophage response to lipopolysaccharide. These results identify the CSF-1R-regulatedmiR-21 network thatmodulatesmacrophage polarization.

Original languageEnglish (US)
Pages (from-to)e1-e13
JournalBlood
Volume125
Issue number8
DOIs
StatePublished - Feb 19 2015

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Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor
Macrophages
MicroRNAs
Phenotype
Monocytes
Macrophage Colony-Stimulating Factor Receptors
Chemical activation
Polarization
Cell signaling
Macrophage Activation
Peritoneal Macrophages
Systems Analysis
Computational Biology
Intraperitoneal Injections
Transcriptome
Bioinformatics
Cell Movement
Lipopolysaccharides
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Colony stimulating factor-1 receptor signaling networks inhibit mouse macrophage inflammatory responses by induction of microRNA-21. / Caescu, Cristina I.; Guo, Xingyi; Tesfa, Lydia; Bhagat, Tushar D.; Verma, Amit K.; Zheng, Deyou; Stanley, E. Richard.

In: Blood, Vol. 125, No. 8, 19.02.2015, p. e1-e13.

Research output: Contribution to journalArticle

Caescu, Cristina I. ; Guo, Xingyi ; Tesfa, Lydia ; Bhagat, Tushar D. ; Verma, Amit K. ; Zheng, Deyou ; Stanley, E. Richard. / Colony stimulating factor-1 receptor signaling networks inhibit mouse macrophage inflammatory responses by induction of microRNA-21. In: Blood. 2015 ; Vol. 125, No. 8. pp. e1-e13.
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