Cold-induced mitochondrial uncoupling and expression of chicken UCP and ANT mRNA in chicken skeletal muscle

Masaaki Toyomizu, Masatoshi Ueda, Shinichi Sato, Yoshinori Seki, Kan Sato, Yukio Akiba

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Although bird species studied thus far have no distinct brown adipose tissue (BAT) or a related thermogenic tissue, there is now strong evidence that non-shivering mechanisms in birds may play an important role during cold exposure. Recently, increased expression of the duckling homolog of the avian uncoupling protein (avUCP) was demonstrated in cold-acclimated ducklings [Raimbault et al., Biochem. J. 353 (2001) 441-444]. Among the mitochondrial anion carriers, roles for the ATP/ADP antiporter (ANT) as well as UCP variants in thermogenesis are proposed. The present experiments were conducted (i) to examine the effects of cold acclimation on the fatty acid-induced uncoupling of oxidative phosphorylation in skeletal muscle mitochondria and (ii) to clone the cDNA of UCP and ANT homologs from chicken skeletal muscle and study differences compared to controls in expression levels of their mRNAs in the skeletal muscle of cold-acclimated chickens. The results obtained here show that suppression of palmitate-induced uncoupling by carboxyatractylate was greater in the subsarcolemmal skeletal muscle mitochondria from cold-acclimated chickens than that for control birds. An increase in mRNA levels of avANT and, to lesser degree, of avUCP in the skeletal muscle of cold-acclimated chickens was also found. Taken together, the present studies on cold-acclimated chickens suggest that the simultaneous increments in levels of avANT and avUCP mRNA expression may be involved in the regulation of thermogenesis in skeletal muscle.

Original languageEnglish (US)
Pages (from-to)313-318
Number of pages6
JournalFEBS Letters
Volume529
Issue number2-3
DOIs
StatePublished - Oct 9 2002
Externally publishedYes

Fingerprint

Antiporters
Muscle
Chickens
Skeletal Muscle
Messenger RNA
Birds
Mitochondria
Muscle Mitochondrion
Thermogenesis
Tissue
ATP Translocases Mitochondrial ADP
Palmitates
Brown Adipose Tissue
Acclimatization
Oxidative Phosphorylation
Adenosine Diphosphate
Anions
Fatty Acids
Complementary DNA
Adenosine Triphosphate

Keywords

  • avANT
  • avUCP
  • Cold-acclimated chicken
  • Skeletal muscle
  • Thermogenesis
  • Uncoupling

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cold-induced mitochondrial uncoupling and expression of chicken UCP and ANT mRNA in chicken skeletal muscle. / Toyomizu, Masaaki; Ueda, Masatoshi; Sato, Shinichi; Seki, Yoshinori; Sato, Kan; Akiba, Yukio.

In: FEBS Letters, Vol. 529, No. 2-3, 09.10.2002, p. 313-318.

Research output: Contribution to journalArticle

Toyomizu, Masaaki ; Ueda, Masatoshi ; Sato, Shinichi ; Seki, Yoshinori ; Sato, Kan ; Akiba, Yukio. / Cold-induced mitochondrial uncoupling and expression of chicken UCP and ANT mRNA in chicken skeletal muscle. In: FEBS Letters. 2002 ; Vol. 529, No. 2-3. pp. 313-318.
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