Cloning of the rabbit homologue of mouse 'basigin' and rat 'OX-47'

Kidney cell type-specific expression, and regulation in collecting duct cells

Victor L. Schuster, Run Lu, Naoaki Kanai, Yi Bao, Stuart O. Rosenberg, Dominique Prié, Pierre Ronco, Michael L. Jennings

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Monoclonal antibody '4D4' was generated against a gel-purified 43-50 kDa fraction of rabbit erythrocyte (RBC) ghosts. Immunoblots of rabbit RBCs, skeletal muscle, and kidney, and of a rabbit cortical collecting duct cell line (RC.SV3) yielded broad bands of 30-70 kDa that migrated at ~ 31 kDa after deglycosylation. In kidney sections, 4D4 labeled the basal plasma membranes of the proximal tubule, medullary thick ascending limb of Henle, cortical, medullary, and papillary collecting ducts, and papillary surface epithelium, as well as the lateral membranes of α and β-type intercalated cells. Antibody 4D4 was used to clone a full-length kidney cDNA, which predicted a 31 kDa immunoglobulin-like glycoprotein with high homology to mouse 'gp42' or 'basigin', human 'M6' or 'EMMPRIN', rat 'OX-47' or 'CE-9', and avian 'neurothelin', 'HT7', or '5A11'. When heterologously expressed in HeLa cells, glycosylated immunoreactive protein was expressed at the plasma membrane. In the case of the endogenous protein in RC.SV3 cells, interferon-γ and A23187 decreased, and fetal calf serum increased, steady-state mRNA levels. Thus, this molecule exhibits a high degree of cell type-specific expression in the kidney and undergoes regulation by cytokines and serum in kidney epithelial cells.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1311
Issue number1
DOIs
StatePublished - Mar 27 1996

Fingerprint

CD147 Antigens
Organism Cloning
Rabbits
Kidney
Cell Membrane
Calcimycin
Erythrocyte Membrane
Serum
HeLa Cells
Interferons
Immunoglobulins
Glycoproteins
Skeletal Muscle
Proteins
Extremities
Epithelium
Complementary DNA
Clone Cells
Gels
Epithelial Cells

Keywords

  • Cell adhesion
  • Cytokine
  • Epithelial polarity
  • Immunglobulin superfamily
  • Molecular cloning

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

Cloning of the rabbit homologue of mouse 'basigin' and rat 'OX-47' : Kidney cell type-specific expression, and regulation in collecting duct cells. / Schuster, Victor L.; Lu, Run; Kanai, Naoaki; Bao, Yi; Rosenberg, Stuart O.; Prié, Dominique; Ronco, Pierre; Jennings, Michael L.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1311, No. 1, 27.03.1996, p. 13-19.

Research output: Contribution to journalArticle

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abstract = "Monoclonal antibody '4D4' was generated against a gel-purified 43-50 kDa fraction of rabbit erythrocyte (RBC) ghosts. Immunoblots of rabbit RBCs, skeletal muscle, and kidney, and of a rabbit cortical collecting duct cell line (RC.SV3) yielded broad bands of 30-70 kDa that migrated at ~ 31 kDa after deglycosylation. In kidney sections, 4D4 labeled the basal plasma membranes of the proximal tubule, medullary thick ascending limb of Henle, cortical, medullary, and papillary collecting ducts, and papillary surface epithelium, as well as the lateral membranes of α and β-type intercalated cells. Antibody 4D4 was used to clone a full-length kidney cDNA, which predicted a 31 kDa immunoglobulin-like glycoprotein with high homology to mouse 'gp42' or 'basigin', human 'M6' or 'EMMPRIN', rat 'OX-47' or 'CE-9', and avian 'neurothelin', 'HT7', or '5A11'. When heterologously expressed in HeLa cells, glycosylated immunoreactive protein was expressed at the plasma membrane. In the case of the endogenous protein in RC.SV3 cells, interferon-γ and A23187 decreased, and fetal calf serum increased, steady-state mRNA levels. Thus, this molecule exhibits a high degree of cell type-specific expression in the kidney and undergoes regulation by cytokines and serum in kidney epithelial cells.",
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