Cloning of AIP1, a novel protein that associates with the apoptosis- linked gene ALG-2 in a Ca2+-dependent reaction

Pasquale Vito, Luca Pellegrini, Chantal Guiet, Luciano D'Adamio

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

ALG-2 is a 22-kDa calcium-binding protein necessary for cell death induced by different stimuli in 3DO T-cell hyhridoma. 3DO cell clones depleted of ALG-2 protein exhibit normal caspases activation, suggesting that ALG-2 function is required downstream or is independent of caspase proteases activity for apoptosis to occur. Using the yeast two-hybrid screening system, we have isolated and characterized the mouse cDNA encoding for ALG-2 interacting protein 1 (AIP1), a novel protein that interacts with ALG-2. ALG- 2 and AIP1 colocalize in the cytosol and the presence of calcium is an indispensable requisite for their association. Sequence alignment shows that AIP1 is highly similar to BRO1, a yeast protein related to components of the Pkc1p-MAP kinase cascade. Overexpression of a truncated form of AIP1 protects two different cell types from death induced by trophic factors withdrawal; thus, our data indicate that AIP1 cooperates with ALG-2 in executing the calcium-dependent requirements along the cell death pathway.

Original languageEnglish (US)
Pages (from-to)1533-1540
Number of pages8
JournalJournal of Biological Chemistry
Volume274
Issue number3
DOIs
StatePublished - Jan 15 1999
Externally publishedYes

Fingerprint

Cloning
Organism Cloning
Genes
Apoptosis
Proteins
Cell Death
Cell death
Caspases
Clone cells
Calcium
Two-Hybrid System Techniques
Calcium-Binding Proteins
Fungal Proteins
T-cells
MAP Kinase Signaling System
Sequence Alignment
Cytosol
Yeast
Screening
Peptide Hydrolases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cloning of AIP1, a novel protein that associates with the apoptosis- linked gene ALG-2 in a Ca2+-dependent reaction. / Vito, Pasquale; Pellegrini, Luca; Guiet, Chantal; D'Adamio, Luciano.

In: Journal of Biological Chemistry, Vol. 274, No. 3, 15.01.1999, p. 1533-1540.

Research output: Contribution to journalArticle

Vito, Pasquale ; Pellegrini, Luca ; Guiet, Chantal ; D'Adamio, Luciano. / Cloning of AIP1, a novel protein that associates with the apoptosis- linked gene ALG-2 in a Ca2+-dependent reaction. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 3. pp. 1533-1540.
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