TY - JOUR
T1 - Cloning and sequencing of a 1.3 kb variant of human thyrotropin receptor mRNA lacking the transmembrane domain
AU - Graves, Peter N.
AU - Tomer, Yaron
AU - Davies, Terry F.
N1 - Funding Information:
ACKNOWLEDGMENTS Supported in part by grants DK28243 and DK35674 Erlinda Conception for excellent technical assistance.
PY - 1992/9/16
Y1 - 1992/9/16
N2 - We amplified human thyroidal cDNA using oligonucleotide primers designed to reveal putative human thyrotropin receptor (hTSHR) mRNA variants encoding the extracellular, ligand-binding domain but lacking the transmembrane domain. Whereas the major 4.3 kb hTSHR mRNA species was not amplified to detectable levels, several shorter products were detected. A strongly amplified 1 kb product was cloned and sequenced. It contained coding sequences at the 5′ end which were colinear with exons 1-8 of the hTSHR gene, encoding most of the extracellular domain. This was followed at the 3′ end by additional coding and noncoding information not present in the 4.3 kb transcript. A probe specific for the 5′ end recognized polyadenylated thyroidal transcripts of 4.3, 1.7, and 1.3 kb, indicating the presence of several hTSHR mRNA variants. A probe specific for the 3′ end recognized only the 1.3 kb transcript. The level of the 1.3 kb variant (hTSHR-v1.3 mRNA) was about half that of the 4.3 kb hTSHR mRNA and twice that of the 1.7 kb variant. The presence of a thyroidal mRNA encoding both the signal peptide and ligand-binding region of the hTSHR, but not the seven transmembrane helices, provides the potential to produce soluble receptors which could play important roles in thyroid physiology and/or autoimmune thyroid disease.
AB - We amplified human thyroidal cDNA using oligonucleotide primers designed to reveal putative human thyrotropin receptor (hTSHR) mRNA variants encoding the extracellular, ligand-binding domain but lacking the transmembrane domain. Whereas the major 4.3 kb hTSHR mRNA species was not amplified to detectable levels, several shorter products were detected. A strongly amplified 1 kb product was cloned and sequenced. It contained coding sequences at the 5′ end which were colinear with exons 1-8 of the hTSHR gene, encoding most of the extracellular domain. This was followed at the 3′ end by additional coding and noncoding information not present in the 4.3 kb transcript. A probe specific for the 5′ end recognized polyadenylated thyroidal transcripts of 4.3, 1.7, and 1.3 kb, indicating the presence of several hTSHR mRNA variants. A probe specific for the 3′ end recognized only the 1.3 kb transcript. The level of the 1.3 kb variant (hTSHR-v1.3 mRNA) was about half that of the 4.3 kb hTSHR mRNA and twice that of the 1.7 kb variant. The presence of a thyroidal mRNA encoding both the signal peptide and ligand-binding region of the hTSHR, but not the seven transmembrane helices, provides the potential to produce soluble receptors which could play important roles in thyroid physiology and/or autoimmune thyroid disease.
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U2 - 10.1016/0006-291X(92)91315-H
DO - 10.1016/0006-291X(92)91315-H
M3 - Article
C2 - 1530609
AN - SCOPUS:0026802536
SN - 0006-291X
VL - 187
SP - 1135
EP - 1143
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -