Cloning and Identification of a Novel Human RNPC3 Gene That Encodes a Protein with Two RRM Domains and Is Expressed in the Cell Nucleus

Enpeng Zhao, Jinsong Li, Yi Xie, Wei Jin, Zhen Zhang, Jinzhong Chen, Li Zeng, Gang Yin, Ji Qian, Hai Wu, Kang Ying, Robert Chunhua Zhao, Yu Min Mao

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The RNA recognition motifs (RRM) domain is one of the most common eukaryotic protein folds. Proteins containing RRM domains function in important steps of posttranscriptional regulation of gene expression and are involved in processing and transport of mRNA precursors. Here we describe the cloning and characterization of a novel human RNPC3 gene containing two RNA recognition motifs. The 1870 bp cDNA encodes a protein with 517 amino acids. It also contains two bipartite nuclear targeting sequences, which is important for nuclear targeting for proteins, especially those functioning in the cell nucleus. The GFP location of the RNPC3 gene product shows that this protein is located in the cell nucleus. RT-PCR reveals that it is abundantly expressed in kidney and pancreas.

Original languageEnglish (US)
Pages (from-to)315-323
Number of pages9
JournalBiochemical Genetics
Volume41
Issue number9-10
DOIs
StatePublished - Oct 1 2003

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Keywords

  • Bipartite nuclear targeting sequence
  • Human RNPC3
  • RNA binding
  • RRM domain

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Zhao, E., Li, J., Xie, Y., Jin, W., Zhang, Z., Chen, J., Zeng, L., Yin, G., Qian, J., Wu, H., Ying, K., Zhao, R. C., & Mao, Y. M. (2003). Cloning and Identification of a Novel Human RNPC3 Gene That Encodes a Protein with Two RRM Domains and Is Expressed in the Cell Nucleus. Biochemical Genetics, 41(9-10), 315-323. https://doi.org/10.1023/B:BIGI.0000006032.04031.d0