Cloning and gene expression of hexokinase I and II in the chicken skeletal muscle

Yoshinori Seki, Kan Sato, Tatsuyoshi Kono, Yukio Akiba

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To elucidate the distinctive regulatory mechanisms of glucose homeostasis in the skeletal muscles of broiler chickens, hexokinase (HK)I and HKII gene expressions were characterized by northern blot analysis. Chicken HKI and HKII full-length cDNA sequences were identified and the deduced amino acid sequences were 87.4 and 85.9% identical to that of human HKI and HKII, respectively. Using reverse transcription-polymerase chain reaction analysis, chicken HKI mRNA was detected in the skeletal muscle, brain, heart, kidney and testis. Chicken HKII mRNA was highly expressed in the skeletal muscle and heart, whereas a lower expression was observed in the liver, brain and kidney. Fasting for 48 h or refeeding for 24 h in chickens significantly increased the skeletal muscle HKI and HKII mRNA levels in contrast to unchanged or decreased expressions in mammals. The present findings suggest that HKI and HKII have a unique role in the glucose homeostasis regulatory mechanism of chicken skeletal muscles and their role in chickens is not similar to that in mammals.

Original languageEnglish (US)
Pages (from-to)491-497
Number of pages7
JournalAnimal Science Journal
Volume76
Issue number5
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Hexokinase
hexokinase
skeletal muscle
Organism Cloning
molecular cloning
Chickens
Skeletal Muscle
chickens
Gene Expression
gene expression
homeostasis
Messenger RNA
heart
kidneys
Mammals
mammals
Homeostasis
brain
glucose
refeeding

Keywords

  • Chicken
  • Glucose homeostasis
  • Hexokinase
  • Hyperglycemia
  • Skeletal muscle

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • veterinary(all)

Cite this

Cloning and gene expression of hexokinase I and II in the chicken skeletal muscle. / Seki, Yoshinori; Sato, Kan; Kono, Tatsuyoshi; Akiba, Yukio.

In: Animal Science Journal, Vol. 76, No. 5, 10.2005, p. 491-497.

Research output: Contribution to journalArticle

Seki, Yoshinori ; Sato, Kan ; Kono, Tatsuyoshi ; Akiba, Yukio. / Cloning and gene expression of hexokinase I and II in the chicken skeletal muscle. In: Animal Science Journal. 2005 ; Vol. 76, No. 5. pp. 491-497.
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