Cloning and characterization of the 5′ upstream region of the human primary response gene EGR-3

J. Liu, M. Nau, L. Grogan, P. Brown, C. Allegra, E. Chu, J. Wright

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Egr-3 is an immediate-early primary response gene encoding a zinc finger transcription factor. We cloned the human Egr-3 genomic locus including greater than 1100 bp of the 5′ flanking region and analyzed this region for putative cis-acting elements. The GC-rich promoter forms part of a representative CpG island that extends into the genomic locus. The Egr-3 promoter contains a region of TATA homology located 25bp upstream from a major transcriptional start site. One serum response element and two variant Egr consensus sequences were identified. Features that distinguished Egr-3 from other human Egr gene promoters included the presence of at least five E-box motifs and a retinoblastoma response element. In addition, an overlapping tandem repeat of 16 GC-rich nonamers was identified in the flanking region that may represent a novel regulatory region for this primary response gene. Reporter constructs coupled with Egr-3 flanking sequences in sense and antisense orientation were tested in transient transfection assays. The functional activity of the Egr-3 regulatory region was position-specific. Deletional analysis in serum stimulated embryonic lung fibroblasts identified that the major elements responsible for growth-induced Egr-3 expression are located within the first 378 bp upstream of the major transcription start site. Analysis of the human Egr-3 genomic locus revealed a complex regulatory organization with significant differences from other Egr genes. These findings may provide insights into the expression of Egr-3 in normal and neoplastic tissues.

Original languageEnglish (US)
Pages (from-to)13-20
Number of pages8
JournalMolecular Biology Today
Volume2
Issue number2
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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