Cloning and characterisation of mmc-1, a microfilarial-specific gene, from Brugia pahangi

Richard Emes, Fiona Thompson, Joyce Moore, Xingxing Zang, Eileen Devaney

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Nine differentially expressed genes were cloned from Brugia pahangi in a screen which sought to identify cDNAs that were differentially expressed between the microfilariae from the mammalian host and the mosquito vector. One gene (mmc-1), that was up-regulated in mammalian-derived microfilariae, was characterised in detail. RT-PCR analysis demonstrated that mmc-1 was specific to the microfilarial stage of the life cycle and was not transcribed by developing microfilariae in utero, but only following the release of the microfilariae from the adult female. Analysis of DNA from other filarial worms suggested that mmc-1 may be a Brugia-specific gene. Using serum samples from individuals exposed to Brugia malayi infection, it was shown that MMC-1 was specifically recognised by antibodies of the IgG3 subclass. mmc-1 has no homologues in the data bases and its function in the parasite is unknown.

Original languageEnglish (US)
Pages (from-to)415-424
Number of pages10
JournalInternational Journal for Parasitology
Volume32
Issue number4
DOIs
Publication statusPublished - Feb 28 2002
Externally publishedYes

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Keywords

  • Differential screen
  • Stage-specific gene expression

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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