Cloning and characterisation of mmc-1, a microfilarial-specific gene, from Brugia pahangi

Richard Emes; Fiona Thompson; Joyce Moore; Xingxing Zang; Eileen Devaney

doi:10.1016/S0020-7519(02)00003-6

Cloning and characterisation of mmc-1, a microfilarial-specific gene, from Brugia pahangi

Richard Emes, Fiona Thompson, Joyce Moore, Xingxing Zang, Eileen Devaney

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Nine differentially expressed genes were cloned from Brugia pahangi in a screen which sought to identify cDNAs that were differentially expressed between the microfilariae from the mammalian host and the mosquito vector. One gene (mmc-1), that was up-regulated in mammalian-derived microfilariae, was characterised in detail. RT-PCR analysis demonstrated that mmc-1 was specific to the microfilarial stage of the life cycle and was not transcribed by developing microfilariae in utero, but only following the release of the microfilariae from the adult female. Analysis of DNA from other filarial worms suggested that mmc-1 may be a Brugia-specific gene. Using serum samples from individuals exposed to Brugia malayi infection, it was shown that MMC-1 was specifically recognised by antibodies of the IgG3 subclass. mmc-1 has no homologues in the data bases and its function in the parasite is unknown.

Original language	English (US)
Pages (from-to)	415-424
Number of pages	10
Journal	International Journal for Parasitology
Volume	32
Issue number	4
DOIs	https://doi.org/10.1016/S0020-7519(02)00003-6
State	Published - 2002
Externally published	Yes

Keywords

Differential screen
Stage-specific gene expression

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0020-7519(02)00003-6

Cite this

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title = "Cloning and characterisation of mmc-1, a microfilarial-specific gene, from Brugia pahangi",

abstract = "Nine differentially expressed genes were cloned from Brugia pahangi in a screen which sought to identify cDNAs that were differentially expressed between the microfilariae from the mammalian host and the mosquito vector. One gene (mmc-1), that was up-regulated in mammalian-derived microfilariae, was characterised in detail. RT-PCR analysis demonstrated that mmc-1 was specific to the microfilarial stage of the life cycle and was not transcribed by developing microfilariae in utero, but only following the release of the microfilariae from the adult female. Analysis of DNA from other filarial worms suggested that mmc-1 may be a Brugia-specific gene. Using serum samples from individuals exposed to Brugia malayi infection, it was shown that MMC-1 was specifically recognised by antibodies of the IgG3 subclass. mmc-1 has no homologues in the data bases and its function in the parasite is unknown.",

keywords = "Differential screen, Stage-specific gene expression",

author = "Richard Emes and Fiona Thompson and Joyce Moore and Xingxing Zang and Eileen Devaney",

note = "Funding Information: This study was funded by grants from the Wellcome Trust and the MRC. R.E. was supported by a PhD studentship from the MRC. We would like to acknowledge the technical support of Isla Wheatley and we thank Mark Blaxter and David Guiliano (University of Edinburgh) for the EST cluster analysis and Steven Williams (Smith College) for provision of the W. bancrofti microfilariae cDNA library. Thanks also to the following who contributed genomic DNA from other filarial parasites: Claude Maina (NEB), Jan Bradley (University of Nottingham), William Harnett (Strathclyde University), Judith Allen and Laetitia LeGoff (University of Edinburgh) and Jean-Paul Akue (CIRMF, Gabon) and to Maria Yazdanbakhsh (University of Leiden) for provision of the serum samples. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2002",

doi = "10.1016/S0020-7519(02)00003-6",

language = "English (US)",

volume = "32",

pages = "415--424",

journal = "International Journal for Parasitology",

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AU - Emes, Richard

AU - Thompson, Fiona

AU - Moore, Joyce

AU - Zang, Xingxing

AU - Devaney, Eileen

N1 - Funding Information: This study was funded by grants from the Wellcome Trust and the MRC. R.E. was supported by a PhD studentship from the MRC. We would like to acknowledge the technical support of Isla Wheatley and we thank Mark Blaxter and David Guiliano (University of Edinburgh) for the EST cluster analysis and Steven Williams (Smith College) for provision of the W. bancrofti microfilariae cDNA library. Thanks also to the following who contributed genomic DNA from other filarial parasites: Claude Maina (NEB), Jan Bradley (University of Nottingham), William Harnett (Strathclyde University), Judith Allen and Laetitia LeGoff (University of Edinburgh) and Jean-Paul Akue (CIRMF, Gabon) and to Maria Yazdanbakhsh (University of Leiden) for provision of the serum samples. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2002

Y1 - 2002

N2 - Nine differentially expressed genes were cloned from Brugia pahangi in a screen which sought to identify cDNAs that were differentially expressed between the microfilariae from the mammalian host and the mosquito vector. One gene (mmc-1), that was up-regulated in mammalian-derived microfilariae, was characterised in detail. RT-PCR analysis demonstrated that mmc-1 was specific to the microfilarial stage of the life cycle and was not transcribed by developing microfilariae in utero, but only following the release of the microfilariae from the adult female. Analysis of DNA from other filarial worms suggested that mmc-1 may be a Brugia-specific gene. Using serum samples from individuals exposed to Brugia malayi infection, it was shown that MMC-1 was specifically recognised by antibodies of the IgG3 subclass. mmc-1 has no homologues in the data bases and its function in the parasite is unknown.

AB - Nine differentially expressed genes were cloned from Brugia pahangi in a screen which sought to identify cDNAs that were differentially expressed between the microfilariae from the mammalian host and the mosquito vector. One gene (mmc-1), that was up-regulated in mammalian-derived microfilariae, was characterised in detail. RT-PCR analysis demonstrated that mmc-1 was specific to the microfilarial stage of the life cycle and was not transcribed by developing microfilariae in utero, but only following the release of the microfilariae from the adult female. Analysis of DNA from other filarial worms suggested that mmc-1 may be a Brugia-specific gene. Using serum samples from individuals exposed to Brugia malayi infection, it was shown that MMC-1 was specifically recognised by antibodies of the IgG3 subclass. mmc-1 has no homologues in the data bases and its function in the parasite is unknown.

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KW - Stage-specific gene expression

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Cloning and characterisation of mmc-1, a microfilarial-specific gene, from Brugia pahangi

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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