Circular viral DNA and anomalous junction sequence in PBMC of HIV-infected individuals with no detectable plasma HIV RNA

A. Cara, J. Vargas, Marla J. Keller, S. Jones, A. Mosoian, A. Gurtman, A. Cohen, V. Parkas, F. Wallach, E. Chusid, I. H. Gelman, M. E. Klotman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Closed circular (cc) forms of extrachromosomal HIV DNA are detected in patients with high viral loads; however, it is unclear whether these forms remain if virus replication is suppressed to undetectable levels by combination antiretroviral therapy. A nested primer polymerase chain reaction amplification assay was used to detect the presence of ccDNA containing two long terminal repeat sequences (2-LTR) in PBMC of patients with low or undetectable plasma HIV RNA. Fifty percent of patients with plasma RNA levels <50 copies/ml of blood had detectable 2-LTR DNA. Sequencing of the products identified normal LTR-LTR junctions in the minority of cases with the majority containing anomalies including deletions and insertions. The persistence of HIV ccDNA in patients with no detectable plasma RNA could be consistent with ongoing de novo infection of dividing cells or with stability of this form of DNA in nondividing cells.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalVirology
Volume292
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

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Circular DNA
Viral DNA
HIV
RNA
Terminal Repeat Sequences
DNA
Virus Replication
Viral Load
Polymerase Chain Reaction
Infection
Therapeutics

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Circular viral DNA and anomalous junction sequence in PBMC of HIV-infected individuals with no detectable plasma HIV RNA. / Cara, A.; Vargas, J.; Keller, Marla J.; Jones, S.; Mosoian, A.; Gurtman, A.; Cohen, A.; Parkas, V.; Wallach, F.; Chusid, E.; Gelman, I. H.; Klotman, M. E.

In: Virology, Vol. 292, No. 1, 2002, p. 1-5.

Research output: Contribution to journalArticle

Cara, A, Vargas, J, Keller, MJ, Jones, S, Mosoian, A, Gurtman, A, Cohen, A, Parkas, V, Wallach, F, Chusid, E, Gelman, IH & Klotman, ME 2002, 'Circular viral DNA and anomalous junction sequence in PBMC of HIV-infected individuals with no detectable plasma HIV RNA', Virology, vol. 292, no. 1, pp. 1-5. https://doi.org/10.1006/viro.2001.1243
Cara, A. ; Vargas, J. ; Keller, Marla J. ; Jones, S. ; Mosoian, A. ; Gurtman, A. ; Cohen, A. ; Parkas, V. ; Wallach, F. ; Chusid, E. ; Gelman, I. H. ; Klotman, M. E. / Circular viral DNA and anomalous junction sequence in PBMC of HIV-infected individuals with no detectable plasma HIV RNA. In: Virology. 2002 ; Vol. 292, No. 1. pp. 1-5.
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AU - Keller, Marla J.

AU - Jones, S.

AU - Mosoian, A.

AU - Gurtman, A.

AU - Cohen, A.

AU - Parkas, V.

AU - Wallach, F.

AU - Chusid, E.

AU - Gelman, I. H.

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