Circular dichroism studies of cobalt substituted lentil lectin

Emilie Stafford; W. David Behnke; Lokesh Bhattacharyya; C. Fred Brewer

doi:10.1016/0006-291X(86)90930-7

Circular dichroism studies of cobalt substituted lentil lectin

Emilie Stafford, W. David Behnke, Lokesh Bhattacharyya, C. Fred Brewer

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A recent method has been developed to effect metal ion substitution at the Mn²⁺ site in the lentil lectin (Bhattacharyya et al. (1984) Biochem. Biophys. Res. Commun. 124, 857-862). We report here the preparation of cobalt substituted lentil lectin, containing Co²⁺ at the S1 site and Ca²⁺ at the S2 site. The cobalt derivative possesses full saccharide binding activity and can be used for spectroscopic studies. The near UV and visible CD spectra of the derivative are shown, and its spectral properties are compared with various cobalt complexes of concanavalin A.

Original language	English (US)
Pages (from-to)	438-444
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	136
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(86)90930-7
State	Published - Apr 14 1986

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(86)90930-7

Cite this

@article{73e55be333bf4ed4aa719480f1fe2fb8,

title = "Circular dichroism studies of cobalt substituted lentil lectin",

abstract = "A recent method has been developed to effect metal ion substitution at the Mn2+ site in the lentil lectin (Bhattacharyya et al. (1984) Biochem. Biophys. Res. Commun. 124, 857-862). We report here the preparation of cobalt substituted lentil lectin, containing Co2+ at the S1 site and Ca2+ at the S2 site. The cobalt derivative possesses full saccharide binding activity and can be used for spectroscopic studies. The near UV and visible CD spectra of the derivative are shown, and its spectral properties are compared with various cobalt complexes of concanavalin A.",

author = "Emilie Stafford and Behnke, {W. David} and Lokesh Bhattacharyya and Brewer, {C. Fred}",

note = "Funding Information: acknowledges helpful conversations with Drs. Seymour Koenig and Rodney Brown, III, of the IBM T.J. Watson Research Center. The present work was supported in part by Grant CA 16054, awarded to C.F.B. by the National Cancer Institute, Department of Health, Education and Welfare,",

year = "1986",

month = apr,

day = "14",

doi = "10.1016/0006-291X(86)90930-7",

language = "English (US)",

volume = "136",

pages = "438--444",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Circular dichroism studies of cobalt substituted lentil lectin

AU - Stafford, Emilie

AU - Behnke, W. David

AU - Bhattacharyya, Lokesh

AU - Brewer, C. Fred

N1 - Funding Information: acknowledges helpful conversations with Drs. Seymour Koenig and Rodney Brown, III, of the IBM T.J. Watson Research Center. The present work was supported in part by Grant CA 16054, awarded to C.F.B. by the National Cancer Institute, Department of Health, Education and Welfare,

PY - 1986/4/14

Y1 - 1986/4/14

N2 - A recent method has been developed to effect metal ion substitution at the Mn2+ site in the lentil lectin (Bhattacharyya et al. (1984) Biochem. Biophys. Res. Commun. 124, 857-862). We report here the preparation of cobalt substituted lentil lectin, containing Co2+ at the S1 site and Ca2+ at the S2 site. The cobalt derivative possesses full saccharide binding activity and can be used for spectroscopic studies. The near UV and visible CD spectra of the derivative are shown, and its spectral properties are compared with various cobalt complexes of concanavalin A.

AB - A recent method has been developed to effect metal ion substitution at the Mn2+ site in the lentil lectin (Bhattacharyya et al. (1984) Biochem. Biophys. Res. Commun. 124, 857-862). We report here the preparation of cobalt substituted lentil lectin, containing Co2+ at the S1 site and Ca2+ at the S2 site. The cobalt derivative possesses full saccharide binding activity and can be used for spectroscopic studies. The near UV and visible CD spectra of the derivative are shown, and its spectral properties are compared with various cobalt complexes of concanavalin A.

UR - http://www.scopus.com/inward/record.url?scp=0023048479&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023048479&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(86)90930-7

DO - 10.1016/0006-291X(86)90930-7

M3 - Article

C2 - 3707581

AN - SCOPUS:0023048479

SN - 0006-291X

VL - 136

SP - 438

EP - 444

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Circular dichroism studies of cobalt substituted lentil lectin

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this