Circular dichroism studies of cobalt substituted lentil lectin

Emilie Stafford, W. David Behnke, Lokesh Bhattacharyya, C. Fred Brewer

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A recent method has been developed to effect metal ion substitution at the Mn2+ site in the lentil lectin (Bhattacharyya et al. (1984) Biochem. Biophys. Res. Commun. 124, 857-862). We report here the preparation of cobalt substituted lentil lectin, containing Co2+ at the S1 site and Ca2+ at the S2 site. The cobalt derivative possesses full saccharide binding activity and can be used for spectroscopic studies. The near UV and visible CD spectra of the derivative are shown, and its spectral properties are compared with various cobalt complexes of concanavalin A.

Original languageEnglish (US)
Pages (from-to)438-444
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume136
Issue number1
DOIs
StatePublished - Apr 14 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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