Chromosomal distribution, localization and expression of the human endogenous retrovirus ERV9

A. C. Svensson, T. Raudsepp, C. Larsson, Antonio Di Cristofano, B. Chowdhary, G. La Mantia, L. Rask, Göran Andersson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

ERV9 is a class I family of human endogenous retroviral sequences. Somatic cell hybrid genomic hybridization experiments using a mono-chromosomal panel indicate the presence of approximately 120 ERV9 loci in the human genome distributed on most chromosomes. Fluorescence in situ hybridization (FISH) using an ERV9 cDNA probe containing gag, pol and env sequences, verified this observation and a consistent signal was found at the chromosome region 11q13.3 → q13.5. By analysis of a panel of radiation hybrids, an ERV9 locus was mapped to within a 300-kbp region at the chromosome site 11q13. The marker cCLGW567 and the locus MAP3K11/D11S546 centromeric and telomeric flanked it, respectively. Northern blot analysis, using an ERV9 LTR probe, indicated that most normal tissues examined expressed low abundant ERV9 LTR driven mRNAs of various sizes. The most prominent expression was found in adrenal glands and testis. However, the level of expression varied in the same tissues among different individuals indicating that ERV9 mRNA expression probably is inducible in certain tissues or at various cell stages.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalCytogenetics and Cell Genetics
Volume92
Issue number1-2
StatePublished - 2001
Externally publishedYes

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Endogenous Retroviruses
Chromosomes
Radiation Hybrid Mapping
Nucleic Acid Hybridization
Messenger RNA
Hybrid Cells
Human Genome
Adrenal Glands
Fluorescence In Situ Hybridization
Northern Blotting
Testis
Complementary DNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Svensson, A. C., Raudsepp, T., Larsson, C., Di Cristofano, A., Chowdhary, B., La Mantia, G., ... Andersson, G. (2001). Chromosomal distribution, localization and expression of the human endogenous retrovirus ERV9. Cytogenetics and Cell Genetics, 92(1-2), 89-96.

Chromosomal distribution, localization and expression of the human endogenous retrovirus ERV9. / Svensson, A. C.; Raudsepp, T.; Larsson, C.; Di Cristofano, Antonio; Chowdhary, B.; La Mantia, G.; Rask, L.; Andersson, Göran.

In: Cytogenetics and Cell Genetics, Vol. 92, No. 1-2, 2001, p. 89-96.

Research output: Contribution to journalArticle

Svensson, AC, Raudsepp, T, Larsson, C, Di Cristofano, A, Chowdhary, B, La Mantia, G, Rask, L & Andersson, G 2001, 'Chromosomal distribution, localization and expression of the human endogenous retrovirus ERV9', Cytogenetics and Cell Genetics, vol. 92, no. 1-2, pp. 89-96.
Svensson, A. C. ; Raudsepp, T. ; Larsson, C. ; Di Cristofano, Antonio ; Chowdhary, B. ; La Mantia, G. ; Rask, L. ; Andersson, Göran. / Chromosomal distribution, localization and expression of the human endogenous retrovirus ERV9. In: Cytogenetics and Cell Genetics. 2001 ; Vol. 92, No. 1-2. pp. 89-96.
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AU - La Mantia, G.

AU - Rask, L.

AU - Andersson, Göran

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