TY - JOUR
T1 - Chromophore chemistry of fluorescent proteins controlled by light
AU - Shcherbakova, Daria M.
AU - Verkhusha, Vladislav V.
N1 - Funding Information:
This work was supported by grants from the Academy of Finland ( 263371 and 266992 ) and the US National Institutes of Health ( GM073913 , CA164468 and EB013571 ).
PY - 2014/6
Y1 - 2014/6
N2 - Recent progress in molecular engineering of genetically encoded probes whose spectral properties are controlled with light, such as photoactivatable, photoswitchable and reversibly switchable fluorescent proteins, has brought the new possibilities to bioimaging and super-resolution microscopy. The development of modern photoconvertible proteins is linked to the studies of light-induced chromophore transformations. Here, we summarize the current view on the chromophore chemistry in the photocontrollable fluorescent proteins. We describe both the fundamental principles and the specific molecular mechanisms underlying the irreversible and reversible chromophore photoconversions. We discuss advancements in super-resolution microscopy that became possible due to the engineering of new protein phenotypes and understanding of their chromophore transformations.
AB - Recent progress in molecular engineering of genetically encoded probes whose spectral properties are controlled with light, such as photoactivatable, photoswitchable and reversibly switchable fluorescent proteins, has brought the new possibilities to bioimaging and super-resolution microscopy. The development of modern photoconvertible proteins is linked to the studies of light-induced chromophore transformations. Here, we summarize the current view on the chromophore chemistry in the photocontrollable fluorescent proteins. We describe both the fundamental principles and the specific molecular mechanisms underlying the irreversible and reversible chromophore photoconversions. We discuss advancements in super-resolution microscopy that became possible due to the engineering of new protein phenotypes and understanding of their chromophore transformations.
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U2 - 10.1016/j.cbpa.2014.04.010
DO - 10.1016/j.cbpa.2014.04.010
M3 - Review article
C2 - 24819887
AN - SCOPUS:84899963630
SN - 1367-5931
VL - 20
SP - 60
EP - 68
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
IS - 1
ER -