Chromophore chemistry of fluorescent proteins controlled by light

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30 Citations (Scopus)

Abstract

Recent progress in molecular engineering of genetically encoded probes whose spectral properties are controlled with light, such as photoactivatable, photoswitchable and reversibly switchable fluorescent proteins, has brought the new possibilities to bioimaging and super-resolution microscopy. The development of modern photoconvertible proteins is linked to the studies of light-induced chromophore transformations. Here, we summarize the current view on the chromophore chemistry in the photocontrollable fluorescent proteins. We describe both the fundamental principles and the specific molecular mechanisms underlying the irreversible and reversible chromophore photoconversions. We discuss advancements in super-resolution microscopy that became possible due to the engineering of new protein phenotypes and understanding of their chromophore transformations.

Original languageEnglish (US)
Pages (from-to)60-68
Number of pages9
JournalCurrent Opinion in Chemical Biology
Volume20
Issue number1
DOIs
StatePublished - 2014

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Chromophores
Light
Microscopy
Protein Engineering
Microscopic examination
Proteins
Phenotype

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry

Cite this

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abstract = "Recent progress in molecular engineering of genetically encoded probes whose spectral properties are controlled with light, such as photoactivatable, photoswitchable and reversibly switchable fluorescent proteins, has brought the new possibilities to bioimaging and super-resolution microscopy. The development of modern photoconvertible proteins is linked to the studies of light-induced chromophore transformations. Here, we summarize the current view on the chromophore chemistry in the photocontrollable fluorescent proteins. We describe both the fundamental principles and the specific molecular mechanisms underlying the irreversible and reversible chromophore photoconversions. We discuss advancements in super-resolution microscopy that became possible due to the engineering of new protein phenotypes and understanding of their chromophore transformations.",
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