Cholinergic modulation of purinergic and GABAergic co-transmission at in vitro hypothalamic synapses

Young-Hwan Jo, Lorna W. Role

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The lateral hypothalamus (LH) is an important center for the integration of autonomic and limbic information and is implicated in the modulation of visceral motor and sensory pathways, including those underlying feeding and arousal behaviors. LH neurons in vitro release both ATP and GABA. The control of ATP and GABA co-transmission in LH may underlie the participation of LH in basic aspects of arousal and reinforcement. LH neurons receive cholinergic input from the pedunculopontine and laterodorsal tegmental nuclei as well as from cholinergic interneurons within the LH per se. This study presents evidence for nicotinic acetyl-choline receptor (nAChR)-mediated enhancement of GABAergic, but not of purinergic, transmission despite the co-transmission of ATP and GABA at LH synapses in vitro. Facilitation of GABAergic transmission by nicotine is inhibited by antagonists of (αβ)*-containing nAChRs, but is unaffected by an α7-selective antagonist, consistent with a nAChR-mediated enhancement of GABA release mediated by non-α7-containing nAChRs. Activation of muscarinic ACh receptors enhances the release of ATP while concomitantly depressing GABAergic transmission. The independent modulation of ATP/GABAergic transmission may provide a new level of synaptic flexibility in which individual neurons utilize more than one neurotransmitter but retain independent control over their synaptic activity.

Original languageEnglish (US)
Pages (from-to)2501-2508
Number of pages8
JournalJournal of Neurophysiology
Volume88
Issue number5
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Lateral Hypothalamic Area
Synapses
Cholinergic Agents
Adenosine Triphosphate
gamma-Aminobutyric Acid
Choline
Arousal
Pedunculopontine Tegmental Nucleus
Efferent Pathways
Neurons
Cholinergic Neurons
Cholinergic Receptors
Muscarinic Receptors
Interneurons
Feeding Behavior
In Vitro Techniques
Nicotine
Neurotransmitter Agents

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Cholinergic modulation of purinergic and GABAergic co-transmission at in vitro hypothalamic synapses. / Jo, Young-Hwan; Role, Lorna W.

In: Journal of Neurophysiology, Vol. 88, No. 5, 01.11.2002, p. 2501-2508.

Research output: Contribution to journalArticle

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