Cholesterol absorption is mainly regulated by the jejunal and ileal ATP-binding cassette sterol efflux transporters Abcg5 and Abcg8 in mice

Li Ping Duan, Helen H. Wang, David Q.H. Wang

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

In the present study, we investigated whether intestinal sterol efflux transporters Abcg5 and Abcg8 play a major role in determining variations in cholesterol (Ch) absorption efficiency, and we compared the physiological functions of the duodenal, jejunal, and ileal Abcg5 and Abcg8 on absorption of Ch and sitostanol in inbred mice challenged with various amounts of Ch, sitostanol, by. drophilic, or hydrophobic bile acids. We found that Abcg5 and Abcg8 in the jejunum and ileum, but not in the duodenum, were main factors in determining, in part, variations in Ch absorption efficiency. The jejunal and ileal Abcg5 and Abcg8 played a major regulatory role in response to high dietary cholesterol and were more sensitive in the regulation of Ch absorption when compared with sitostanol absorption. These results, combined with different sterol uptake rates, suggest that the absorption efficiency of Ch and sitostanol is determined by the net results between influx and efflux of intraluminal Ch and sitostanol molecules crossing the apical membrane of the enterocyte. Hydrophilic and hydrophobic bile acids influenced Ch absorption through mediating Ch solubilization and its physical-chemical state within the small intestinal lumen. We conclude that Ch absorption is mainly regulated by the jejunal and ieal Abcg5 and Abcg8 in mice.

Original languageEnglish (US)
Pages (from-to)1312-1323
Number of pages12
JournalJournal of Lipid Research
Volume45
Issue number7
DOIs
StatePublished - Jul 1 2004
Externally publishedYes

Fingerprint

Sterols
Adenosine Triphosphate
Cholesterol
Bile Acids and Salts
Dietary Cholesterol
Enterocytes
Jejunum
Ileum
Duodenum
stigmastanol
Membranes
Molecules

Keywords

  • Bile salt
  • Chylomicron
  • Genetics
  • Intestinal lipid uptake
  • Lymph
  • Micelle
  • Nutrition
  • Phospholipid

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Cholesterol absorption is mainly regulated by the jejunal and ileal ATP-binding cassette sterol efflux transporters Abcg5 and Abcg8 in mice. / Duan, Li Ping; Wang, Helen H.; Wang, David Q.H.

In: Journal of Lipid Research, Vol. 45, No. 7, 01.07.2004, p. 1312-1323.

Research output: Contribution to journalArticle

@article{83b9335a5469446abcc0e6c2ce92aa67,
title = "Cholesterol absorption is mainly regulated by the jejunal and ileal ATP-binding cassette sterol efflux transporters Abcg5 and Abcg8 in mice",
abstract = "In the present study, we investigated whether intestinal sterol efflux transporters Abcg5 and Abcg8 play a major role in determining variations in cholesterol (Ch) absorption efficiency, and we compared the physiological functions of the duodenal, jejunal, and ileal Abcg5 and Abcg8 on absorption of Ch and sitostanol in inbred mice challenged with various amounts of Ch, sitostanol, by. drophilic, or hydrophobic bile acids. We found that Abcg5 and Abcg8 in the jejunum and ileum, but not in the duodenum, were main factors in determining, in part, variations in Ch absorption efficiency. The jejunal and ileal Abcg5 and Abcg8 played a major regulatory role in response to high dietary cholesterol and were more sensitive in the regulation of Ch absorption when compared with sitostanol absorption. These results, combined with different sterol uptake rates, suggest that the absorption efficiency of Ch and sitostanol is determined by the net results between influx and efflux of intraluminal Ch and sitostanol molecules crossing the apical membrane of the enterocyte. Hydrophilic and hydrophobic bile acids influenced Ch absorption through mediating Ch solubilization and its physical-chemical state within the small intestinal lumen. We conclude that Ch absorption is mainly regulated by the jejunal and ieal Abcg5 and Abcg8 in mice.",
keywords = "Bile salt, Chylomicron, Genetics, Intestinal lipid uptake, Lymph, Micelle, Nutrition, Phospholipid",
author = "Duan, {Li Ping} and Wang, {Helen H.} and Wang, {David Q.H.}",
year = "2004",
month = "7",
day = "1",
doi = "10.1194/jlr.M400030-JLR200",
language = "English (US)",
volume = "45",
pages = "1312--1323",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "7",

}

TY - JOUR

T1 - Cholesterol absorption is mainly regulated by the jejunal and ileal ATP-binding cassette sterol efflux transporters Abcg5 and Abcg8 in mice

AU - Duan, Li Ping

AU - Wang, Helen H.

AU - Wang, David Q.H.

PY - 2004/7/1

Y1 - 2004/7/1

N2 - In the present study, we investigated whether intestinal sterol efflux transporters Abcg5 and Abcg8 play a major role in determining variations in cholesterol (Ch) absorption efficiency, and we compared the physiological functions of the duodenal, jejunal, and ileal Abcg5 and Abcg8 on absorption of Ch and sitostanol in inbred mice challenged with various amounts of Ch, sitostanol, by. drophilic, or hydrophobic bile acids. We found that Abcg5 and Abcg8 in the jejunum and ileum, but not in the duodenum, were main factors in determining, in part, variations in Ch absorption efficiency. The jejunal and ileal Abcg5 and Abcg8 played a major regulatory role in response to high dietary cholesterol and were more sensitive in the regulation of Ch absorption when compared with sitostanol absorption. These results, combined with different sterol uptake rates, suggest that the absorption efficiency of Ch and sitostanol is determined by the net results between influx and efflux of intraluminal Ch and sitostanol molecules crossing the apical membrane of the enterocyte. Hydrophilic and hydrophobic bile acids influenced Ch absorption through mediating Ch solubilization and its physical-chemical state within the small intestinal lumen. We conclude that Ch absorption is mainly regulated by the jejunal and ieal Abcg5 and Abcg8 in mice.

AB - In the present study, we investigated whether intestinal sterol efflux transporters Abcg5 and Abcg8 play a major role in determining variations in cholesterol (Ch) absorption efficiency, and we compared the physiological functions of the duodenal, jejunal, and ileal Abcg5 and Abcg8 on absorption of Ch and sitostanol in inbred mice challenged with various amounts of Ch, sitostanol, by. drophilic, or hydrophobic bile acids. We found that Abcg5 and Abcg8 in the jejunum and ileum, but not in the duodenum, were main factors in determining, in part, variations in Ch absorption efficiency. The jejunal and ileal Abcg5 and Abcg8 played a major regulatory role in response to high dietary cholesterol and were more sensitive in the regulation of Ch absorption when compared with sitostanol absorption. These results, combined with different sterol uptake rates, suggest that the absorption efficiency of Ch and sitostanol is determined by the net results between influx and efflux of intraluminal Ch and sitostanol molecules crossing the apical membrane of the enterocyte. Hydrophilic and hydrophobic bile acids influenced Ch absorption through mediating Ch solubilization and its physical-chemical state within the small intestinal lumen. We conclude that Ch absorption is mainly regulated by the jejunal and ieal Abcg5 and Abcg8 in mice.

KW - Bile salt

KW - Chylomicron

KW - Genetics

KW - Intestinal lipid uptake

KW - Lymph

KW - Micelle

KW - Nutrition

KW - Phospholipid

UR - http://www.scopus.com/inward/record.url?scp=3042522984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042522984&partnerID=8YFLogxK

U2 - 10.1194/jlr.M400030-JLR200

DO - 10.1194/jlr.M400030-JLR200

M3 - Article

C2 - 15102882

AN - SCOPUS:3042522984

VL - 45

SP - 1312

EP - 1323

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 7

ER -