Chinifur, a Selective Inhibitor and "Subversive Substrate" for Trypanosoma congolense Trypanothione Reductase

Narimantas Cenas, Daiva Bironaite, Egle Dickancaite, Zilvinas Anusevicius, Jonas Sarlauskas, John S. Blanchard

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Nitrofurans with aromatic and heterocyclic substituents inhibit Trypanosoma congolense trypanothione reductase (TR) and yeast glutathione reductase (GR), acting as uncompetitive inhibitors vs. NADPH and noncompetitive or uncompetitive inhibitors vs. disulfide substrate. Many of these compounds inhibited trypanothione reductase more efficiently than glutathione reductase. Chinifur (2-(5′-nitro(furo-2′-yl)-ethene-1-yl)-4(N, N-diethylamino)-1- methyl-but-1-yl-arninocarbonyl-4-quinoline) was the most selective inhibitor of, and free radical-generating substrate for, trypanothione reductase (Ki = 4.5 μm, TN = 3 s-1, TN/Km = 3.2 × 104 M-1 s-1), only weakly inhibiting glutathione reductase (Ki = 100 μm). These findings point to the importance of hydrophobic interactions in the design of redox active heteroaromatic compounds acting as selective inhibitors of, and "subversive substrates" for, trypanothione reductase.

Original languageEnglish (US)
Pages (from-to)224-229
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume204
Issue number1
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Chinifur, a Selective Inhibitor and "Subversive Substrate" for Trypanosoma congolense Trypanothione Reductase'. Together they form a unique fingerprint.

  • Cite this