Abstract
Nitric oxide (NO) is a cell-signaling molecule involved in a number of physiological and pathophysiological processes. Modification of cysteine residues by NO (or NO metabolites), that is S-nitrosation, changes the function of a broad spectrum of proteins. This reaction represents an important post-translational modification that transduces NO-dependent signals. However, the detection and quantification of S-nitrosation in biological samples remain a challenge mainly because of the lability of S-nitrosation products: S-nitrosothiols (SNO). In this review we summarize recent developments of the methods to detect S-nitrosation. Our focus is on the methods which can be used to directly conjugate the site(s) of S-nitrosation.
Original language | English (US) |
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Pages (from-to) | 32-37 |
Number of pages | 6 |
Journal | Current Opinion in Chemical Biology |
Volume | 15 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2011 |
Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry