Abstract
Nitric oxide (NO) is a cell-signaling molecule involved in a number of physiological and pathophysiological processes. Modification of cysteine residues by NO (or NO metabolites), that is S-nitrosation, changes the function of a broad spectrum of proteins. This reaction represents an important post-translational modification that transduces NO-dependent signals. However, the detection and quantification of S-nitrosation in biological samples remain a challenge mainly because of the lability of S-nitrosation products: S-nitrosothiols (SNO). In this review we summarize recent developments of the methods to detect S-nitrosation. Our focus is on the methods which can be used to directly conjugate the site(s) of S-nitrosation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 32-37 |
| Number of pages | 6 |
| Journal | Current Opinion in Chemical Biology |
| Volume | 15 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2011 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
Cite this
Chemical methods to detect S-nitrosation. / Wang, Hua; Xian, Ming.
In: Current Opinion in Chemical Biology, Vol. 15, No. 1, 02.2011, p. 32-37.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemical methods to detect S-nitrosation
AU - Wang, Hua
AU - Xian, Ming
PY - 2011/2
Y1 - 2011/2
N2 - Nitric oxide (NO) is a cell-signaling molecule involved in a number of physiological and pathophysiological processes. Modification of cysteine residues by NO (or NO metabolites), that is S-nitrosation, changes the function of a broad spectrum of proteins. This reaction represents an important post-translational modification that transduces NO-dependent signals. However, the detection and quantification of S-nitrosation in biological samples remain a challenge mainly because of the lability of S-nitrosation products: S-nitrosothiols (SNO). In this review we summarize recent developments of the methods to detect S-nitrosation. Our focus is on the methods which can be used to directly conjugate the site(s) of S-nitrosation.
AB - Nitric oxide (NO) is a cell-signaling molecule involved in a number of physiological and pathophysiological processes. Modification of cysteine residues by NO (or NO metabolites), that is S-nitrosation, changes the function of a broad spectrum of proteins. This reaction represents an important post-translational modification that transduces NO-dependent signals. However, the detection and quantification of S-nitrosation in biological samples remain a challenge mainly because of the lability of S-nitrosation products: S-nitrosothiols (SNO). In this review we summarize recent developments of the methods to detect S-nitrosation. Our focus is on the methods which can be used to directly conjugate the site(s) of S-nitrosation.
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UR - http://www.scopus.com/inward/citedby.url?scp=79851509433&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2010.10.006
DO - 10.1016/j.cbpa.2010.10.006
M3 - Article
VL - 15
SP - 32
EP - 37
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
SN - 1367-5931
IS - 1
ER -