Chemical methods to detect S-nitrosation

Hua Wang, Ming Xian

Research output: Contribution to journalReview article

44 Scopus citations

Abstract

Nitric oxide (NO) is a cell-signaling molecule involved in a number of physiological and pathophysiological processes. Modification of cysteine residues by NO (or NO metabolites), that is S-nitrosation, changes the function of a broad spectrum of proteins. This reaction represents an important post-translational modification that transduces NO-dependent signals. However, the detection and quantification of S-nitrosation in biological samples remain a challenge mainly because of the lability of S-nitrosation products: S-nitrosothiols (SNO). In this review we summarize recent developments of the methods to detect S-nitrosation. Our focus is on the methods which can be used to directly conjugate the site(s) of S-nitrosation.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalCurrent Opinion in Chemical Biology
Volume15
Issue number1
DOIs
StatePublished - Feb 1 2011

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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