Chemical induction of cardiac differentiation in P19 embryonal carcinoma stem cells

Jasmin, David C. Spray, Antonio Carlos Campos De Carvalho, Rosalia Mendez-Otero

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

P19 cells, a pluripotent cell line derived from a teratocarcinoma induced in C3H/HeHa mice, have been widely used as a model system to study cardiac differentiation. We have used these cells to evaluate the extent to which exposure to DMSO and/or cardiogenol C for 4 days in suspension culture enhanced their differentiation into cardiomyocytes. Cardiac differentiation was assessed by observing beating clusters and further confirmed using immunocytochemical, biochemical, and pharmacological approaches. The presence of functional gap junctions in differentiated P19 cells was identified through calcium wave analyses. Proliferation rate and cell death were analyzed by BrdU incorporation and activated caspase-3 immunodetection, respectively. Beating clusters of differentiated P19 cells were only found in cultures treated with DMSO. In addition, groups treated with DMSO up-regulated cardiac troponin-T expression. However, when DMSO was used together with cardiogenol C the up-regulation was less than that with DMSO alone, ∼1.5 times. Moreover, P19 cells cultured in DMSO or DMSO plus 0.25 M cardiogenol C had lower proliferation rates and higher numbers of activated caspase-3-positive cells. In summary, using several methodological approaches we have demonstrated that DMSO can induce cardiac differentiation of P19 cells but that cardiogenol C does not.

Original languageEnglish (US)
Pages (from-to)403-411
Number of pages9
JournalStem Cells and Development
Volume19
Issue number3
DOIs
StatePublished - Mar 1 2010

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Embryonal Carcinoma Stem Cells
Dimethyl Sulfoxide
Caspase 3
Teratocarcinoma
Troponin T
Calcium Signaling
Inbred C3H Mouse
Gap Junctions
Bromodeoxyuridine
Cardiac Myocytes
Cell Differentiation
Cultured Cells
Suspensions
Cell Death
Up-Regulation
Pharmacology
Cell Line
cardiogenol C

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

Chemical induction of cardiac differentiation in P19 embryonal carcinoma stem cells. / Jasmin, ; Spray, David C.; De Carvalho, Antonio Carlos Campos; Mendez-Otero, Rosalia.

In: Stem Cells and Development, Vol. 19, No. 3, 01.03.2010, p. 403-411.

Research output: Contribution to journalArticle

Jasmin, ; Spray, David C. ; De Carvalho, Antonio Carlos Campos ; Mendez-Otero, Rosalia. / Chemical induction of cardiac differentiation in P19 embryonal carcinoma stem cells. In: Stem Cells and Development. 2010 ; Vol. 19, No. 3. pp. 403-411.
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