Characterization of tumor-induced platelet aggregation: The role of immunorelated GPIb and GPIIb IIIa expression by MCF-7 breast cancer cells

Leslie Oleksowicz, Zbigniew Mrowiec, Edward Schwartz, Manoochehr Khorshidi, Janice P. Dutcher, Elena Puszkin

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

Tumor cell induced platelet aggregation is thought to be an early step in the metastatic process. Here we show that platelet aggregation induced by MCF-7 cells is mediated, in part, through an ADP-dependent mechanism based on inhibition of aggregation by pretreatment of the tumor cells with apyrase and the identification of ADP in tumor cell-free supernatants by HPLC. By applying immunocytochemical and flow cytometric techniques, we demonstrate that platelet immunorelated glycoproteins, GPIb, GPIIb IIIa, GPIb IX, and the integrin αv subunit are expressed on the surface of MCF-7 cells. The expression of an immunorelated GPIb was further confirmed by immunoblot and autoradiography of 125I-labelled MCF-7 cells. MCF-7 cell immunoblot preparations demonstrated one major protein reactive to an anti-GPIbα MoAb under nonreduced conditions with a molecular weight of 200 kD and two major proteins reactive with the anti-GPIbα MoAb under reduced conditions with molecular weights of 92 kD and 38 kD. Platelet aggregation is inhibited by preincubating the MCF-7 cells with antibodies to GPIb and GPIIb IIIa. These findings document expression of adhesive glycoproteins by MCF-7 cancer cells and suggest that these receptors, together with ADP, play a role in tumor induced platelet aggregation.

Original languageEnglish (US)
Pages (from-to)261-274
Number of pages14
JournalThrombosis Research
Volume79
Issue number3
DOIs
StatePublished - Aug 1 1995

Keywords

  • MCF-7 cultured tumor cells
  • adenosine diphosphate
  • breast carcinoma
  • platelet aggregation
  • platelet glycoproteins

ASJC Scopus subject areas

  • Hematology

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