Characterization of the VIER F-BOX PROTEINE genes from arabidopsis reveals their importance for plant growth and development

Katja M. Schwager, Luz Irina A Calderon-Villalobos, Esther M N Dohmann, Björn C. Willige, Stephan Knierer, Carola Nill, Claus Schwechheimer

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

E3 ubiquitin ligases (E3s) target proteins for degradation by the 26S proteasome. In SKP1/CDC53/F-box protein-type E3s, substrate specificity is conferred by the interchangeable F-box protein subunit. The vast majority of the 694 F-box proteins encoded by the Arabidopsis thaliana genome remain to be understood. We characterize the VIER F-BOX PROTEINE (VFB; German for FOUR F-BOX PROTEINS) genes from Arabidopsis that belong to subfamily C of the Arabidopsis F-box protein superfamily. This subfamily also includes the F-box proteins TRANSPORT INHIBITOR RESPONSE1 (TIR1)/AUXIN SIGNALING F-BOX (AFB) proteins and EIN3 BINDING F-BOX proteins, which regulate auxin and ethylene responses, respectively. We show that loss of VFB function causes delayed plant growth and reduced lateral root formation. We find that the expression of a number of auxin-responsive genes and the activity of DR5:β-glucuronidase, a reporter for auxin reponse, are reduced in the vfb mutants. This finding correlates with an increase in the abundance of an AUXIN/INDOLE-3-ACETIC ACID repressor. However, we also find that auxin responses are not affected in the vfb mutants and that a representative VFB family member, VFB2, cannot functionally complement the tir1-1 mutant. We therefore exclude the possibility that VFBs are functional orthologs of TIR1/AFB proteins.

Original languageEnglish (US)
Pages (from-to)1163-1178
Number of pages16
JournalPlant Cell
Volume19
Issue number4
DOIs
StatePublished - Apr 2007
Externally publishedYes

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F-Box Proteins
F-box proteins
Plant Development
Indoleacetic Acids
Growth and Development
Arabidopsis
plant development
growth and development
Genes
auxins
plant growth
genes
mutants
Arabidopsis Proteins
Complement C1
ubiquitin-protein ligase
Ubiquitin-Protein Ligases
Glucuronidase
Protein Subunits
proteasome endopeptidase complex

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Schwager, K. M., Calderon-Villalobos, L. I. A., Dohmann, E. M. N., Willige, B. C., Knierer, S., Nill, C., & Schwechheimer, C. (2007). Characterization of the VIER F-BOX PROTEINE genes from arabidopsis reveals their importance for plant growth and development. Plant Cell, 19(4), 1163-1178. https://doi.org/10.1105/tpc.105.040675

Characterization of the VIER F-BOX PROTEINE genes from arabidopsis reveals their importance for plant growth and development. / Schwager, Katja M.; Calderon-Villalobos, Luz Irina A; Dohmann, Esther M N; Willige, Björn C.; Knierer, Stephan; Nill, Carola; Schwechheimer, Claus.

In: Plant Cell, Vol. 19, No. 4, 04.2007, p. 1163-1178.

Research output: Contribution to journalArticle

Schwager, KM, Calderon-Villalobos, LIA, Dohmann, EMN, Willige, BC, Knierer, S, Nill, C & Schwechheimer, C 2007, 'Characterization of the VIER F-BOX PROTEINE genes from arabidopsis reveals their importance for plant growth and development', Plant Cell, vol. 19, no. 4, pp. 1163-1178. https://doi.org/10.1105/tpc.105.040675
Schwager KM, Calderon-Villalobos LIA, Dohmann EMN, Willige BC, Knierer S, Nill C et al. Characterization of the VIER F-BOX PROTEINE genes from arabidopsis reveals their importance for plant growth and development. Plant Cell. 2007 Apr;19(4):1163-1178. https://doi.org/10.1105/tpc.105.040675
Schwager, Katja M. ; Calderon-Villalobos, Luz Irina A ; Dohmann, Esther M N ; Willige, Björn C. ; Knierer, Stephan ; Nill, Carola ; Schwechheimer, Claus. / Characterization of the VIER F-BOX PROTEINE genes from arabidopsis reveals their importance for plant growth and development. In: Plant Cell. 2007 ; Vol. 19, No. 4. pp. 1163-1178.
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