Characterization of the targeting, binding, and phosphorylation site domains of an a kinase anchor protein and a myristoylated alanine-rich C kinase substrate-like analog that are encoded by a single gene

Edmund A. Rossi, Zhuo Li, Hui Feng, Charles S. Rubin

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A novel Drosophila A kinase anchor protein, Drosophila A kinase anchor protein 200 (DAKAP200), is predicted to be involved in routing, mediating, and integrating signals carried by cAMP, Ca2+, and diacylglycerol (Li, Z., Rossi, E. A., Hoheisel, J. D., Kalderon, D., and Rubin, C. S. (1999) J. Biol. Chem. 274, 27191-27200). Experiments designed to assess this hypothesis now (a) establish the function, boundaries and identity of critical amino acids of the protein kinase AII (PKAII) tethering site of DAKAP200; (b) demonstrate that residues 119-148 mediate binding with Ca2+-calmodulin and F-actin; (c) show that a polybasic region of DAKAP200 is a substrate for protein kinase C; (d) reveal that phosphorylation of the polybasic domain regulates affinity for F-actin and Ca2+-calmodulin; and (e) indicate that DAKAP200 is myristoylated and that this modification promotes targeting of DAKAP200 to plasma membrane. ΔAKAP200, a second product of the DAKAP200 gene, cannot tether PKAII. However, ΔAKAP200 is myristoylated and contains a phosphorylation site domain that binds Ca2+-calmodulin and F-actin. An atypical amino acid composition, a high level of negative charge, exceptional thermostability, unusual hydrodynamic properties, properties of the phosphorylation site domain, and a calculated M(r) of 38,000 suggest that ΔAKAP200 is a new member of the myristoylated alanine-rich C kinase substrate protein family. DAKAP200 is a potentially mobile, chimeric A kinase anchor protein-myristoylated alanine-rich C kinase substrate protein that may facilitate localized reception and targeted transmission of signals carried by cAMP, Ca2+, and diacylglycerol.

Original languageEnglish (US)
Pages (from-to)27201-27210
Number of pages10
JournalJournal of Biological Chemistry
Volume274
Issue number38
DOIs
StatePublished - Sep 17 1999

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Phosphorylation
Anchors
Protein Kinases
Phosphotransferases
Genes
Binding Sites
A Kinase Anchor Proteins
Calmodulin
Proteins
Actins
Diglycerides
Drosophila Proteins
Amino Acids
Hydrodynamics
Cell membranes
Drosophila Akap200 protein
myristoylated alanine-rich C kinase substrate
Protein Kinase C
Cell Membrane
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of the targeting, binding, and phosphorylation site domains of an a kinase anchor protein and a myristoylated alanine-rich C kinase substrate-like analog that are encoded by a single gene. / Rossi, Edmund A.; Li, Zhuo; Feng, Hui; Rubin, Charles S.

In: Journal of Biological Chemistry, Vol. 274, No. 38, 17.09.1999, p. 27201-27210.

Research output: Contribution to journalArticle

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abstract = "A novel Drosophila A kinase anchor protein, Drosophila A kinase anchor protein 200 (DAKAP200), is predicted to be involved in routing, mediating, and integrating signals carried by cAMP, Ca2+, and diacylglycerol (Li, Z., Rossi, E. A., Hoheisel, J. D., Kalderon, D., and Rubin, C. S. (1999) J. Biol. Chem. 274, 27191-27200). Experiments designed to assess this hypothesis now (a) establish the function, boundaries and identity of critical amino acids of the protein kinase AII (PKAII) tethering site of DAKAP200; (b) demonstrate that residues 119-148 mediate binding with Ca2+-calmodulin and F-actin; (c) show that a polybasic region of DAKAP200 is a substrate for protein kinase C; (d) reveal that phosphorylation of the polybasic domain regulates affinity for F-actin and Ca2+-calmodulin; and (e) indicate that DAKAP200 is myristoylated and that this modification promotes targeting of DAKAP200 to plasma membrane. ΔAKAP200, a second product of the DAKAP200 gene, cannot tether PKAII. However, ΔAKAP200 is myristoylated and contains a phosphorylation site domain that binds Ca2+-calmodulin and F-actin. An atypical amino acid composition, a high level of negative charge, exceptional thermostability, unusual hydrodynamic properties, properties of the phosphorylation site domain, and a calculated M(r) of 38,000 suggest that ΔAKAP200 is a new member of the myristoylated alanine-rich C kinase substrate protein family. DAKAP200 is a potentially mobile, chimeric A kinase anchor protein-myristoylated alanine-rich C kinase substrate protein that may facilitate localized reception and targeted transmission of signals carried by cAMP, Ca2+, and diacylglycerol.",
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AB - A novel Drosophila A kinase anchor protein, Drosophila A kinase anchor protein 200 (DAKAP200), is predicted to be involved in routing, mediating, and integrating signals carried by cAMP, Ca2+, and diacylglycerol (Li, Z., Rossi, E. A., Hoheisel, J. D., Kalderon, D., and Rubin, C. S. (1999) J. Biol. Chem. 274, 27191-27200). Experiments designed to assess this hypothesis now (a) establish the function, boundaries and identity of critical amino acids of the protein kinase AII (PKAII) tethering site of DAKAP200; (b) demonstrate that residues 119-148 mediate binding with Ca2+-calmodulin and F-actin; (c) show that a polybasic region of DAKAP200 is a substrate for protein kinase C; (d) reveal that phosphorylation of the polybasic domain regulates affinity for F-actin and Ca2+-calmodulin; and (e) indicate that DAKAP200 is myristoylated and that this modification promotes targeting of DAKAP200 to plasma membrane. ΔAKAP200, a second product of the DAKAP200 gene, cannot tether PKAII. However, ΔAKAP200 is myristoylated and contains a phosphorylation site domain that binds Ca2+-calmodulin and F-actin. An atypical amino acid composition, a high level of negative charge, exceptional thermostability, unusual hydrodynamic properties, properties of the phosphorylation site domain, and a calculated M(r) of 38,000 suggest that ΔAKAP200 is a new member of the myristoylated alanine-rich C kinase substrate protein family. DAKAP200 is a potentially mobile, chimeric A kinase anchor protein-myristoylated alanine-rich C kinase substrate protein that may facilitate localized reception and targeted transmission of signals carried by cAMP, Ca2+, and diacylglycerol.

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