Characterization of a targeting motif for a flagellar membrane protein in Leishmania enriettii

Erik L. Snapp, Scott M. Landfear

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The surface membranes of eukaryotic flagella and cilia are contiguous with the plasma membrane. Despite the absence of obvious physical structures that could form a barrier between the two membrane domains, the lipid and protein compositions of flagella and cilia are distinct rest of the cell surface membrane. We have exploited a flagellar glucose transporter parasitic protozoan Leishmania enriettii as a model system to characterize the first targeting motif for a flagellar membrane protein in any eukaryotic organism. In this study, we demonstrate that the flagellar membrane-targeting motif is recognized by several species of Leishmania. Previously, we demonstrated that the 130 amino acid NH2-terminal cytoplasmic domain of isoform 1 glucose transporter was sufficient to target a nonflagellar integral membrane protein into the flagellar membrane. We have now determined that an essential flagellar targeting signal is located between amino acids 20 and 35 of the NH2-terminal domain. We have further analyzed the role of specific amino acids in this region by alanine replacement mutagenesis and determined that single amino acid substitutions did not abrogate targeting to the flagellar membrane. However, individual mutations located within a cluster of five contiguous amino acids, RTGTT, conferred differences in the degree of targeting to the flagellar membrane and the flagellar pocket, implying a role for these residues in the mechanism of flagellar trafficking.

Original languageEnglish (US)
Pages (from-to)29543-29548
Number of pages6
JournalJournal of Biological Chemistry
Volume274
Issue number41
DOIs
StatePublished - Oct 8 1999
Externally publishedYes

Fingerprint

Leishmania enriettii
Membrane Proteins
Membranes
Amino Acids
Facilitative Glucose Transport Proteins
Flagella
Cilia
Cell Membrane
Leishmania
Amino Acid Substitution
Membrane Lipids
Mutagenesis
Alanine
Protein Isoforms
Cell membranes
Mutation
Substitution reactions
Lipids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of a targeting motif for a flagellar membrane protein in Leishmania enriettii. / Snapp, Erik L.; Landfear, Scott M.

In: Journal of Biological Chemistry, Vol. 274, No. 41, 08.10.1999, p. 29543-29548.

Research output: Contribution to journalArticle

Snapp, Erik L. ; Landfear, Scott M. / Characterization of a targeting motif for a flagellar membrane protein in Leishmania enriettii. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 41. pp. 29543-29548.
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