Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene

Zhong Guo Hu; Ye Chen; Qin Zhou; Xiao Yan Lv; Zheng Zhang; Yi Dong Wang; Yan Xiao; Hong Guo; Yu Hang liu; Rui Zhi Tan; Qin Wei Li; Guo Hui Bian; Yu Quan Wei

doi:10.1016/j.bbrc.2007.10.146

Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene

Zhong Guo Hu, Ye Chen, Qin Zhou, Xiao Yan Lv, Zheng Zhang, Yi Dong Wang, Yan Xiao, Hong Guo, Yu Hang liu, Rui Zhi Tan, Qin Wei Li, Guo Hui Bian, Yu Quan Wei

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

SH3 domain binding protein 5 like (xSH3BP5L) gene encodes a protein that is a new found member of SH3 domain binding protein family which has been implicated at multiple levels of biological functions. Here, we have characterized Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene in the development of Xenopus laevis. Transcripts of xSH3BP5L were detected at all stages of development and in numerous adult tissues. Whole-mount in situ hybridization demonstrated that xSH3BP5L is expressed at the animal pole from stage-2 onward. Interestingly, translational inhibition of xSH3BP5L using antisense morpholino oligonucleotides (MOs) and overexpression of xSH3BP5L in Xenopus embryos resulted in failed or delayed blastopore closure. Taken together, these data suggested that xSH3BP5L is required for normal embryogenesis of blastopore closure in X. laevis.

Original language	English (US)
Pages (from-to)	214-220
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	365
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2007.10.146
State	Published - Jan 11 2008
Externally published	Yes

Keywords

Development
SH3 domain binding protein 5 like gene
Xenopus laevis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2007.10.146

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title = "Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene",

abstract = "SH3 domain binding protein 5 like (xSH3BP5L) gene encodes a protein that is a new found member of SH3 domain binding protein family which has been implicated at multiple levels of biological functions. Here, we have characterized Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene in the development of Xenopus laevis. Transcripts of xSH3BP5L were detected at all stages of development and in numerous adult tissues. Whole-mount in situ hybridization demonstrated that xSH3BP5L is expressed at the animal pole from stage-2 onward. Interestingly, translational inhibition of xSH3BP5L using antisense morpholino oligonucleotides (MOs) and overexpression of xSH3BP5L in Xenopus embryos resulted in failed or delayed blastopore closure. Taken together, these data suggested that xSH3BP5L is required for normal embryogenesis of blastopore closure in X. laevis.",

keywords = "Development, SH3 domain binding protein 5 like gene, Xenopus laevis",

author = "Hu, {Zhong Guo} and Ye Chen and Qin Zhou and Lv, {Xiao Yan} and Zheng Zhang and Wang, {Yi Dong} and Yan Xiao and Hong Guo and liu, {Yu Hang} and Tan, {Rui Zhi} and Li, {Qin Wei} and Bian, {Guo Hui} and Wei, {Yu Quan}",

note = "Funding Information: This work was supported by a grant of the National Key Basic Research Program of China to Qin Zhou (2005CB522506) and a grant of the National Key Basic Research Program of China to academician Yuquan Wei (2004CB518800). Dr. Qin Zhou was a recipient of the Initial Foundation of M.O.E. for Returned Overseas Students and a scholarship of the Creative Foundation of Sichuan University. The work was also supported by a grant of the S&T Bureau of Sichuan Province to Qin Zhou. The expert assistance of Prof. Xianming Mo is greatly acknowledged. We thank Prof. Alexander F. Schier for critically reading the manuscript.",

year = "2008",

month = jan,

day = "11",

doi = "10.1016/j.bbrc.2007.10.146",

language = "English (US)",

volume = "365",

pages = "214--220",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene

AU - Hu, Zhong Guo

AU - Chen, Ye

AU - Zhou, Qin

AU - Lv, Xiao Yan

AU - Zhang, Zheng

AU - Wang, Yi Dong

AU - Xiao, Yan

AU - Guo, Hong

AU - liu, Yu Hang

AU - Tan, Rui Zhi

AU - Li, Qin Wei

AU - Bian, Guo Hui

AU - Wei, Yu Quan

N1 - Funding Information: This work was supported by a grant of the National Key Basic Research Program of China to Qin Zhou (2005CB522506) and a grant of the National Key Basic Research Program of China to academician Yuquan Wei (2004CB518800). Dr. Qin Zhou was a recipient of the Initial Foundation of M.O.E. for Returned Overseas Students and a scholarship of the Creative Foundation of Sichuan University. The work was also supported by a grant of the S&T Bureau of Sichuan Province to Qin Zhou. The expert assistance of Prof. Xianming Mo is greatly acknowledged. We thank Prof. Alexander F. Schier for critically reading the manuscript.

PY - 2008/1/11

Y1 - 2008/1/11

N2 - SH3 domain binding protein 5 like (xSH3BP5L) gene encodes a protein that is a new found member of SH3 domain binding protein family which has been implicated at multiple levels of biological functions. Here, we have characterized Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene in the development of Xenopus laevis. Transcripts of xSH3BP5L were detected at all stages of development and in numerous adult tissues. Whole-mount in situ hybridization demonstrated that xSH3BP5L is expressed at the animal pole from stage-2 onward. Interestingly, translational inhibition of xSH3BP5L using antisense morpholino oligonucleotides (MOs) and overexpression of xSH3BP5L in Xenopus embryos resulted in failed or delayed blastopore closure. Taken together, these data suggested that xSH3BP5L is required for normal embryogenesis of blastopore closure in X. laevis.

AB - SH3 domain binding protein 5 like (xSH3BP5L) gene encodes a protein that is a new found member of SH3 domain binding protein family which has been implicated at multiple levels of biological functions. Here, we have characterized Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene in the development of Xenopus laevis. Transcripts of xSH3BP5L were detected at all stages of development and in numerous adult tissues. Whole-mount in situ hybridization demonstrated that xSH3BP5L is expressed at the animal pole from stage-2 onward. Interestingly, translational inhibition of xSH3BP5L using antisense morpholino oligonucleotides (MOs) and overexpression of xSH3BP5L in Xenopus embryos resulted in failed or delayed blastopore closure. Taken together, these data suggested that xSH3BP5L is required for normal embryogenesis of blastopore closure in X. laevis.

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Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene

Abstract

Keywords

ASJC Scopus subject areas

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