Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene

Zhong Guo Hu, Ye Chen, Qin Zhou, Xiao Yan Lv, Zheng Zhang, Yidong Wang, Yan Xiao, Hong Guo, Yu Hang liu, Rui Zhi Tan, Qin Wei Li, Guo Hui Bian, Yu Quan Wei

Research output: Contribution to journalArticle

Abstract

SH3 domain binding protein 5 like (xSH3BP5L) gene encodes a protein that is a new found member of SH3 domain binding protein family which has been implicated at multiple levels of biological functions. Here, we have characterized Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene in the development of Xenopus laevis. Transcripts of xSH3BP5L were detected at all stages of development and in numerous adult tissues. Whole-mount in situ hybridization demonstrated that xSH3BP5L is expressed at the animal pole from stage-2 onward. Interestingly, translational inhibition of xSH3BP5L using antisense morpholino oligonucleotides (MOs) and overexpression of xSH3BP5L in Xenopus embryos resulted in failed or delayed blastopore closure. Taken together, these data suggested that xSH3BP5L is required for normal embryogenesis of blastopore closure in X. laevis.

Original languageEnglish (US)
Pages (from-to)214-220
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume365
Issue number2
DOIs
StatePublished - Jan 11 2008
Externally publishedYes

Fingerprint

Xenopus
Carrier Proteins
Genes
Gastrula
Xenopus laevis
ponsin
Morpholinos
src Homology Domains
Antisense Oligonucleotides
Embryonic Development
In Situ Hybridization
Poles
Animals
Embryonic Structures
Tissue

Keywords

  • Development
  • SH3 domain binding protein 5 like gene
  • Xenopus laevis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene. / Hu, Zhong Guo; Chen, Ye; Zhou, Qin; Lv, Xiao Yan; Zhang, Zheng; Wang, Yidong; Xiao, Yan; Guo, Hong; liu, Yu Hang; Tan, Rui Zhi; Li, Qin Wei; Bian, Guo Hui; Wei, Yu Quan.

In: Biochemical and Biophysical Research Communications, Vol. 365, No. 2, 11.01.2008, p. 214-220.

Research output: Contribution to journalArticle

Hu, ZG, Chen, Y, Zhou, Q, Lv, XY, Zhang, Z, Wang, Y, Xiao, Y, Guo, H, liu, YH, Tan, RZ, Li, QW, Bian, GH & Wei, YQ 2008, 'Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene', Biochemical and Biophysical Research Communications, vol. 365, no. 2, pp. 214-220. https://doi.org/10.1016/j.bbrc.2007.10.146
Hu, Zhong Guo ; Chen, Ye ; Zhou, Qin ; Lv, Xiao Yan ; Zhang, Zheng ; Wang, Yidong ; Xiao, Yan ; Guo, Hong ; liu, Yu Hang ; Tan, Rui Zhi ; Li, Qin Wei ; Bian, Guo Hui ; Wei, Yu Quan. / Characterization of a novel Xenopus SH3 domain binding protein 5 like (xSH3BP5L) gene. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 365, No. 2. pp. 214-220.
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