Characterization of a chemotactic and cytotoxic proteinase from human skin

Victor Bernard Hatcher, Gerald S. Lazarus, Norman Levine, Peter G. Burk, Fred J. Yost Jr.

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A proteinase (EC 3.4.-.-) active at physiological pH has been isolated from human skin utilizing gel filtration and affinity chromatography techniques. The proteinase has a molecular weight of approx. 28 000 and it is inhibited by α2-macroglobulin, α1-antitrypsin, C 1 ̄ inactivator, soybean trypsin inhibitor and diiosopropyl fluorophosphate. Injection of 1 ng of purified proteinase into rabbit skin induces polymorphonuclear leukocyte infiltration of the cutis. Inhibition of enzyme activity with diisopropyl fluorophosphate inhibits the chemotactic effect. Addition of 0.2 μg/ml of purified proteinase to fibroblast cultures kills the cells within minutes. This proteinase may play a significant role in modulating the inflammatory response after cellular injury.

Original languageEnglish (US)
Pages (from-to)160-171
Number of pages12
JournalBBA - Enzymology
Volume483
Issue number1
DOIs
StatePublished - Jul 8 1977

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fluorophosphate
Peptide Hydrolases
Skin
Macroglobulins
Trypsin Inhibitors
Affinity Chromatography
Soybeans
Gel Chromatography
Neutrophils
Cell Culture Techniques
Fibroblasts
Molecular Weight
Rabbits
Injections
Wounds and Injuries
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Characterization of a chemotactic and cytotoxic proteinase from human skin. / Hatcher, Victor Bernard; Lazarus, Gerald S.; Levine, Norman; Burk, Peter G.; J. Yost Jr., Fred.

In: BBA - Enzymology, Vol. 483, No. 1, 08.07.1977, p. 160-171.

Research output: Contribution to journalArticle

Hatcher, Victor Bernard ; Lazarus, Gerald S. ; Levine, Norman ; Burk, Peter G. ; J. Yost Jr., Fred. / Characterization of a chemotactic and cytotoxic proteinase from human skin. In: BBA - Enzymology. 1977 ; Vol. 483, No. 1. pp. 160-171.
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