TY - JOUR
T1 - Characterization and functional studies on rat liver fat-storing cell line and freshly isolated hepatocyte coculture system
AU - Rojkind, Marcos
AU - Novikoff, Phyllis M.
AU - Greenwel, Patricia
AU - Rubin, Joshua
AU - Rojas-Valencia, Luz
AU - De Carvalho, Antonio Campos
AU - Stockert, Richard
AU - Spray, David
AU - Hertzberg, Elliot L.
AU - Wolkoff, Allan W.
PY - 1995/6
Y1 - 1995/6
N2 - We developed and characterized a coculture system composed of a fat- storing cell clone (CFSC-2G) and freshly isolated hepatocytes that can reproduce in vitro some of the physical and functional relationships observed in viva Hepatocytes in the coculture are polarized, are smaller in size than hepatocytes plated on plastic, maintain a cuboidal shape, and have a tendency to form cords. Fat-storing cells, which are initially extended, retract and leave spaces that resemble liver sinusoids. Both celt types in the coculture system are functional for at least two weeks as determined by the expression of high levels of liver-specific protein mRNAs as well as by the production and secretion of liver-specific proteins into the culture medium. The hepatocytes maintain relatively high levels of asialoglycoprotein receptor on their celt surface and form functional gap junctional complexes with fat- storing cells. Hence, this coculture system retains a number of differentiated functions of hepatocytes, making it a useful model to study cell-cell interactions in culture and to analyze regulation of hepatocyte functions.
AB - We developed and characterized a coculture system composed of a fat- storing cell clone (CFSC-2G) and freshly isolated hepatocytes that can reproduce in vitro some of the physical and functional relationships observed in viva Hepatocytes in the coculture are polarized, are smaller in size than hepatocytes plated on plastic, maintain a cuboidal shape, and have a tendency to form cords. Fat-storing cells, which are initially extended, retract and leave spaces that resemble liver sinusoids. Both celt types in the coculture system are functional for at least two weeks as determined by the expression of high levels of liver-specific protein mRNAs as well as by the production and secretion of liver-specific proteins into the culture medium. The hepatocytes maintain relatively high levels of asialoglycoprotein receptor on their celt surface and form functional gap junctional complexes with fat- storing cells. Hence, this coculture system retains a number of differentiated functions of hepatocytes, making it a useful model to study cell-cell interactions in culture and to analyze regulation of hepatocyte functions.
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M3 - Article
C2 - 7778689
AN - SCOPUS:0029002528
SN - 0002-9440
VL - 146
SP - 1508
EP - 1520
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 6
ER -