Characteristics of manganese (Mn) transport in rat brain endothelial (RBE4) cells, an in vitro model of the blood-brain barrier

Vanessa A. Fitsanakis, Greg Piccola, Judy L. Aschner, Michael Aschner

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Manganese (Mn), an essential elemental nutrient, is known to be neurotoxic at high occupational levels. We examined the transport of Mn across a monolayer of rat brain endothelial cell (RBE4) to evaluate whether an electromotive permeability mechanism is responsible for Mn transport across the blood-brain barrier (BBB). The 54Mn2+ apparent permeability and flux showed significant temperature-, energy- and pH-dependence, as well as partial sodium-dependence. Additionally, iron (Fe)-rich and Fe-deficient media significantly increased the apparent permeability of 54Mn 2+. Finally, Mn flux and permeability decreased when RBE4 cells were grown in astrocyte-conditioned media (ACM), compared to standard alpha-media. These data reinforce observations that transport of Mn across the BBB occurs in part through active transport process.

Original languageEnglish (US)
Pages (from-to)60-70
Number of pages11
JournalNeuroToxicology
Volume27
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

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Endothelial cells
Manganese
Blood-Brain Barrier
Rats
Brain
Endothelial Cells
Permeability
Fluxes
Active Biological Transport
Conditioned Culture Medium
Astrocytes
Nutrients
Monolayers
Iron
Sodium
In Vitro Techniques
Food
Temperature

Keywords

  • Astrocyte-conditioned medium
  • Energy-dependence
  • Iron-dependence
  • Proton-dependence
  • Sodium-dependence

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Toxicology

Cite this

Characteristics of manganese (Mn) transport in rat brain endothelial (RBE4) cells, an in vitro model of the blood-brain barrier. / Fitsanakis, Vanessa A.; Piccola, Greg; Aschner, Judy L.; Aschner, Michael.

In: NeuroToxicology, Vol. 27, No. 1, 01.2006, p. 60-70.

Research output: Contribution to journalArticle

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