Characteristics of manganese (Mn) transport in rat brain endothelial (RBE4) cells, an in vitro model of the blood-brain barrier

Vanessa A. Fitsanakis, Greg Piccola, Judy L. Aschner, Michael Aschner

Research output: Contribution to journalArticle

16 Scopus citations


Manganese (Mn), an essential elemental nutrient, is known to be neurotoxic at high occupational levels. We examined the transport of Mn across a monolayer of rat brain endothelial cell (RBE4) to evaluate whether an electromotive permeability mechanism is responsible for Mn transport across the blood-brain barrier (BBB). The 54Mn2+ apparent permeability and flux showed significant temperature-, energy- and pH-dependence, as well as partial sodium-dependence. Additionally, iron (Fe)-rich and Fe-deficient media significantly increased the apparent permeability of 54Mn 2+. Finally, Mn flux and permeability decreased when RBE4 cells were grown in astrocyte-conditioned media (ACM), compared to standard alpha-media. These data reinforce observations that transport of Mn across the BBB occurs in part through active transport process.

Original languageEnglish (US)
Pages (from-to)60-70
Number of pages11
Issue number1
Publication statusPublished - Jan 1 2006



  • Astrocyte-conditioned medium
  • Energy-dependence
  • Iron-dependence
  • Proton-dependence
  • Sodium-dependence

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

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