Characterisation of N-terminal chromogranin A and chromogranin B in mammals by region-specific radioimmunoassays and chromatographic separation methods

M. Stridsberg, R. H. Angeletti, K. B. Helle

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Chromogranin A (CgA) and chromogranin B (CgB) are acidic proteins stored in and released from hormone granules in endocrine and neuroendocrine tissue. The chromogranins are postulated to serve as pro-hormones to generate biologically active peptides, which may influence hormonal release and vascular functions or have antibacterial functions. Although N-terminal and C-terminal regions show some species amino acid hornology; the chromogranins as a whole display considerable interspecies differences, which prevents their use in comparative studies of biological functions. We present four new radioimmunoassays for the measurement of defined N-terminal regions of CgA and CgB. A new radioimmunoassay for measurement of intact bovine CgA has also been developed. With these assays and two previously published ones, we have compared the cross-reactivity of chromogranins from man, cattle, sheep, goat, pig and horse and compared adrenomedullar content and serum levels of CgA from these species. We have also studied the influence of peptide concentrations and the ionic strength of the mobile phase on molecular weight estimations. Assays with antibodies directed against the N-terminal parts of CgA and CgB showed sufficient interspecies cross-reactivity to allow comparative quantification of the circulating levels in man, cattle, sheep, goat, pig and horse. Assays measuring the intact human or bovine CgA were not suitable for comparative purposes in samples from sheep, goat, pig and horse. Molecular interactions between vasostatin immunoreactive material and intact bovine CgA were demonstrated in gel permeation studies, suggesting that conclusions about the degree of N-terminal processing from elution profiles should be made with caution. Reliable interspecies comparison of chromogranins is difficult, but measurements with region-specific assays may be helpful to study concentrations of chromogranins and chromogranin-related peptides.

Original languageEnglish (US)
Pages (from-to)703-714
Number of pages12
JournalJournal of Endocrinology
Volume165
Issue number3
StatePublished - 2000

Fingerprint

Chromogranin B
Chromogranin A
Chromogranins
Radioimmunoassay
Mammals
Goats
Horses
Sheep
Swine
Peptides
Hormones
Osmolar Concentration
Blood Vessels
Molecular Weight
Gels
Amino Acids
Antibodies

ASJC Scopus subject areas

  • Endocrinology

Cite this

Characterisation of N-terminal chromogranin A and chromogranin B in mammals by region-specific radioimmunoassays and chromatographic separation methods. / Stridsberg, M.; Angeletti, R. H.; Helle, K. B.

In: Journal of Endocrinology, Vol. 165, No. 3, 2000, p. 703-714.

Research output: Contribution to journalArticle

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