Changes in the guanine nucleotide-binding proteins, Gi and go, during differentiation of 3T3-L1 cells

Peter Gierschik, Bernice Morrow, Graeme Milligan, Charles Rubin, Allen Spiegel

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Differentiation of 3T3-L1 cells from fibroblasts to adipocytes is accompanied by increased adenylate cyclase response to lipolytic agents. We used pertussis toxin and specific antibodies to measure the inhibitory guanine nucleotide-binding protein, Gi, and the novel G-protein, Go, in membranes from 3T3-L1 cells. Pertussis toxin-dependent labeling of a 39-40 kDa protein showed an initial 30% rise, followed by an 80% fall during differentiation. Immunoblots showed that 3T3-L1 cells contain Go, as well as Gi, and that changes in the former parallel the changes in pertussis toxin labeling. Changes in Gi and Go may contribute to altered adenylate cyclase response during 3T3-L1 cell differentiation.

Original languageEnglish (US)
Pages (from-to)103-106
Number of pages4
JournalFEBS Letters
Volume199
Issue number1
DOIs
StatePublished - Apr 7 1986

Keywords

  • Adenylate cyclase
  • Pertussis toxin
  • Signal transduction

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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