Changes in biocompatibility of dialysis fluid during its dwell in the peritoneal cavity

A. Breborowicz, L. Martis, D. G. Oreopoulos

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: To evaluate the changes in biocompatibility of peritoneal dialysis solutions during intraperitoneal dwell. Design: We studied the effect of the drained dialysates at time 0 and after 30, 60, 120, 240, and 360 minutes of intraperitoneal dwell on the growth of peritoneal mesothelial cells and fibroblasts and the synthesis of proteins by these cells. On one day the patients were dialyzed with glucose-based Dianeal and on alternate days with an amino acid-containing solution based on Travasol. Patients: Dialysates were collected from 4 patients during continuous ambulatory peritoneal dialysis (CAPD) training. Results: Unused dialysis solutions containing glucose or amino acids inhibit growth of mesothelial cells and fibroblasts. Dialysates obtained after 30 or 60 minutes of intraperitoneal dwell support the growth of these cells in away similar to 10% fetal calf serum, but dialysates drained after a longer dwell of 120-360 minutes had a stronger effect on growth of these cells than did serum. All glucose-based dialysates stimulate the synthesis of collagen in mesothelial cells, whereas they reduce the synthesis of non-collagen proteins. All glucose-based dialysates reduce the synthesis of collagen and non-collagen proteins in fibroblasts compared with the production of these proteins in the presence of serum. Conclusion: Changes in the properties of the dialysis solutions during their intraperitoneal dwells do not seem to increase their biocompatibility. Indeed, excessive mitogenic effect and the stimulation of collagen synthesis of the dialysates may induce pathological changes in the peritoneum.

Original languageEnglish (US)
Pages (from-to)152-157
Number of pages6
JournalPeritoneal Dialysis International
Volume15
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Dialysis Solutions
Peritoneal Cavity
Dialysis
Glucose
Collagen
Fibroblasts
Growth
Proteins
Serum
Amino Acids
Continuous Ambulatory Peritoneal Dialysis
Peritoneum
Peritoneal Dialysis

Keywords

  • Biocompatibility
  • Fibroblasts
  • Mesothelium
  • Peritoneal dialysis

ASJC Scopus subject areas

  • Nephrology

Cite this

Changes in biocompatibility of dialysis fluid during its dwell in the peritoneal cavity. / Breborowicz, A.; Martis, L.; Oreopoulos, D. G.

In: Peritoneal Dialysis International, Vol. 15, No. 3, 1995, p. 152-157.

Research output: Contribution to journalArticle

Breborowicz, A, Martis, L & Oreopoulos, DG 1995, 'Changes in biocompatibility of dialysis fluid during its dwell in the peritoneal cavity', Peritoneal Dialysis International, vol. 15, no. 3, pp. 152-157.
Breborowicz, A. ; Martis, L. ; Oreopoulos, D. G. / Changes in biocompatibility of dialysis fluid during its dwell in the peritoneal cavity. In: Peritoneal Dialysis International. 1995 ; Vol. 15, No. 3. pp. 152-157.
@article{3fe4323fa09a411eae3d721572b5c267,
title = "Changes in biocompatibility of dialysis fluid during its dwell in the peritoneal cavity",
abstract = "Objective: To evaluate the changes in biocompatibility of peritoneal dialysis solutions during intraperitoneal dwell. Design: We studied the effect of the drained dialysates at time 0 and after 30, 60, 120, 240, and 360 minutes of intraperitoneal dwell on the growth of peritoneal mesothelial cells and fibroblasts and the synthesis of proteins by these cells. On one day the patients were dialyzed with glucose-based Dianeal and on alternate days with an amino acid-containing solution based on Travasol. Patients: Dialysates were collected from 4 patients during continuous ambulatory peritoneal dialysis (CAPD) training. Results: Unused dialysis solutions containing glucose or amino acids inhibit growth of mesothelial cells and fibroblasts. Dialysates obtained after 30 or 60 minutes of intraperitoneal dwell support the growth of these cells in away similar to 10{\%} fetal calf serum, but dialysates drained after a longer dwell of 120-360 minutes had a stronger effect on growth of these cells than did serum. All glucose-based dialysates stimulate the synthesis of collagen in mesothelial cells, whereas they reduce the synthesis of non-collagen proteins. All glucose-based dialysates reduce the synthesis of collagen and non-collagen proteins in fibroblasts compared with the production of these proteins in the presence of serum. Conclusion: Changes in the properties of the dialysis solutions during their intraperitoneal dwells do not seem to increase their biocompatibility. Indeed, excessive mitogenic effect and the stimulation of collagen synthesis of the dialysates may induce pathological changes in the peritoneum.",
keywords = "Biocompatibility, Fibroblasts, Mesothelium, Peritoneal dialysis",
author = "A. Breborowicz and L. Martis and Oreopoulos, {D. G.}",
year = "1995",
language = "English (US)",
volume = "15",
pages = "152--157",
journal = "Peritoneal Dialysis International",
issn = "0896-8608",
publisher = "Multimed Inc.",
number = "3",

}

TY - JOUR

T1 - Changes in biocompatibility of dialysis fluid during its dwell in the peritoneal cavity

AU - Breborowicz, A.

AU - Martis, L.

AU - Oreopoulos, D. G.

PY - 1995

Y1 - 1995

N2 - Objective: To evaluate the changes in biocompatibility of peritoneal dialysis solutions during intraperitoneal dwell. Design: We studied the effect of the drained dialysates at time 0 and after 30, 60, 120, 240, and 360 minutes of intraperitoneal dwell on the growth of peritoneal mesothelial cells and fibroblasts and the synthesis of proteins by these cells. On one day the patients were dialyzed with glucose-based Dianeal and on alternate days with an amino acid-containing solution based on Travasol. Patients: Dialysates were collected from 4 patients during continuous ambulatory peritoneal dialysis (CAPD) training. Results: Unused dialysis solutions containing glucose or amino acids inhibit growth of mesothelial cells and fibroblasts. Dialysates obtained after 30 or 60 minutes of intraperitoneal dwell support the growth of these cells in away similar to 10% fetal calf serum, but dialysates drained after a longer dwell of 120-360 minutes had a stronger effect on growth of these cells than did serum. All glucose-based dialysates stimulate the synthesis of collagen in mesothelial cells, whereas they reduce the synthesis of non-collagen proteins. All glucose-based dialysates reduce the synthesis of collagen and non-collagen proteins in fibroblasts compared with the production of these proteins in the presence of serum. Conclusion: Changes in the properties of the dialysis solutions during their intraperitoneal dwells do not seem to increase their biocompatibility. Indeed, excessive mitogenic effect and the stimulation of collagen synthesis of the dialysates may induce pathological changes in the peritoneum.

AB - Objective: To evaluate the changes in biocompatibility of peritoneal dialysis solutions during intraperitoneal dwell. Design: We studied the effect of the drained dialysates at time 0 and after 30, 60, 120, 240, and 360 minutes of intraperitoneal dwell on the growth of peritoneal mesothelial cells and fibroblasts and the synthesis of proteins by these cells. On one day the patients were dialyzed with glucose-based Dianeal and on alternate days with an amino acid-containing solution based on Travasol. Patients: Dialysates were collected from 4 patients during continuous ambulatory peritoneal dialysis (CAPD) training. Results: Unused dialysis solutions containing glucose or amino acids inhibit growth of mesothelial cells and fibroblasts. Dialysates obtained after 30 or 60 minutes of intraperitoneal dwell support the growth of these cells in away similar to 10% fetal calf serum, but dialysates drained after a longer dwell of 120-360 minutes had a stronger effect on growth of these cells than did serum. All glucose-based dialysates stimulate the synthesis of collagen in mesothelial cells, whereas they reduce the synthesis of non-collagen proteins. All glucose-based dialysates reduce the synthesis of collagen and non-collagen proteins in fibroblasts compared with the production of these proteins in the presence of serum. Conclusion: Changes in the properties of the dialysis solutions during their intraperitoneal dwells do not seem to increase their biocompatibility. Indeed, excessive mitogenic effect and the stimulation of collagen synthesis of the dialysates may induce pathological changes in the peritoneum.

KW - Biocompatibility

KW - Fibroblasts

KW - Mesothelium

KW - Peritoneal dialysis

UR - http://www.scopus.com/inward/record.url?scp=0029028379&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029028379&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 152

EP - 157

JO - Peritoneal Dialysis International

JF - Peritoneal Dialysis International

SN - 0896-8608

IS - 3

ER -