Cellular and antibody responses directed against the HIV-1 principal neutralizing domain in HIV-1-infected children

G. Wiseman, Arye Rubinstein, P. Martinez, S. Lambert, Y. Devash, Harris Goldstein

Research output: Contribution to journalArticle

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Abstract

The principal neutralizing domain (PND) for antibody response is located within the V3 variable region of gp120 and can also stimulate T-cell responses. In some adults infected with human immunodeficiency virus (HIV) an HIV-1-specific T-cell response can be detected by demonstrating in vitro proliferation to HIV-1 proteins and peptides. In other HIV-1 infected adults an HIV-1-specific T-cell response can involve interleukin 2 (IL-2) secretion in the absence of T-cell proliferation. To elucidate the T-cell responses to PND in children, we examined the proliferative and the IL-2 secretory responses of peripheral blood lymphocytes from 19 HIV-1-infected children toward a peptide which contained a highly conserved sequence of the principal neutralizing domain of HIV(MN) (PND-MN). Stimulation with PND-MN induced proliferation of lymphocytes from 2 of the children and IL-2 secretion by lymphocytes from 5 of the children. In a 3-month-old infant, the in vitro cellular response to the PND-MN indicated HIV-1 infection prior to the detection p24 antigen in her serum. Although antibodies directed against PND-MN were detected in all but one of the children examined, the presence of high-affinity/avidity antibodies to the PND-MN correlated with the presence of a cellular response to PND-MN. Thus, in HIV-1-infected children an HIV-1 specific T-cell response in the absence of a proliferative response can be assessed by determination of the IL-2 secretory response and correlates with the generation of high-affinity/avidity antibodies.

Original languageEnglish (US)
Pages (from-to)839-845
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume7
Issue number10
StatePublished - 1991

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Antibody Formation
HIV-1
Antibody Affinity
T-Lymphocytes
Interleukin-2
Lymphocytes
HIV
Human Immunodeficiency Virus Proteins
Peptides
Conserved Sequence
Virus Diseases
Neutralizing Antibodies
Cell Proliferation
Antigens
Antibodies
Serum

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Cellular and antibody responses directed against the HIV-1 principal neutralizing domain in HIV-1-infected children. / Wiseman, G.; Rubinstein, Arye; Martinez, P.; Lambert, S.; Devash, Y.; Goldstein, Harris.

In: AIDS Research and Human Retroviruses, Vol. 7, No. 10, 1991, p. 839-845.

Research output: Contribution to journalArticle

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