Cell uncoupling and protein kinase C: correlation in a cell line but not in a differentiated tissue

M. Chanson, R. Bruzzone, David C. Spray, R. Regazzi, P. Meda

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Second messengers have been implicated in the control of communication between cells of various tissues and of a number of cell lines. To assess whether protein kinase C (PKC) is involved in the regulation of gap junctions between primary differentiated cells, we studied the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on PKC translocation and junctional conductance of rat pancreatic exocrine cells. Our results show that although TPA induced the translocation of PKC from a 'cytosolic' to a 'microsomal' fraction within minutes, it failed to block the junctional conductance of acinar cell pairs up to 30 min after application. By contrast, analogous experiments on a liver-derived cell line (WB cells) showed that TPA-induced PKC translocation was paralleled by a marked and irreversible inhibition of intercellular coupling. These results indicate that, in contrast to the effects on transformed or dedifferentiated permanent cell lines, PKC is not involved in gating gap junctional channels between primary differentiated secretory cells of the pancreas.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume255
Issue number5
StatePublished - 1988
Externally publishedYes

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Protein Kinase C
Cells
Tissue
Tetradecanoylphorbol Acetate
Cell Line
Acetates
Acinar Cells
Gap Junctions
Second Messenger Systems
Cell Communication
Liver
Rats
Pancreas
Mitochondrial Uncoupling Proteins
Communication
Experiments

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Cell uncoupling and protein kinase C : correlation in a cell line but not in a differentiated tissue. / Chanson, M.; Bruzzone, R.; Spray, David C.; Regazzi, R.; Meda, P.

In: American Journal of Physiology - Cell Physiology, Vol. 255, No. 5, 1988.

Research output: Contribution to journalArticle

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