@article{9b2f388eda4845dab867a8325f798a0b,
title = "Cell type-specific manipulation with GFP-dependent Cre recombinase",
abstract = "There are many transgenic GFP reporter lines that allow the visualization of specific populations of cells. Using such lines for functional studies requires a method that transforms GFP into a molecule that enables genetic manipulation. We developed a method that exploits GFP for gene manipulation, Cre recombinase dependent on GFP (CRE-DOG), a split component system that uses GFP and its derivatives to directly induce Cre/loxP recombination. Using plasmid electroporation and AAV viral vectors, we delivered CRE-DOG to multiple GFP mouse lines, which led to effective recombination selectively in GFP-labeled cells. Furthermore, CRE-DOG enabled optogenetic control of these neurons. Beyond providing a new set of tools for manipulation of gene expression selectively in GFP + cells, we found that GFP can be used to reconstitute the activity of a protein not known to have a modular structure, suggesting that this strategy might be applicable to a wide range of proteins.",
author = "Tang, {Jonathan C.Y.} and Stephanie Rudolph and Dhande, {Onkar S.} and Abraira, {Victoria E.} and Seungwon Choi and Lapan, {Sylvain W.} and Drew, {Iain R.} and Eugene Drokhlyansky and Huberman, {Andrew D.} and Regehr, {Wade G.} and Cepko, {Constance L.}",
note = "Funding Information: We thank C. Wang of the Z. He laboratory (Boston Children{\textquoteright}s Hospital) for rAAV production (core service supported by grant NEI 5P30EY012196-17), D. Goz and S. Zhao for technical assistance, B. Huang, D. Meijer, and the Cepko, Tabin and Dymecki laboratory members for input on the manuscript, and the Neurobiology Imaging Facility (supported by NINDS P30 Core Center grant NS072030) for consultation and instrument availability. We are grateful to U. Rothbauer and H. Leonhardt (Ludwig Maximilian University of Munich) for providing GBPs. This work was funded by the Howard Hughes Medical Institute (C.L.C.), the Nancy Lurie Marks Foundation (W.G.R.), the Lefler Foundation (W.G.R.), the Knights Templar Eye Foundation (O.S.D.), the McKnight Foundation (A.D.H.), The Pew Charitable Trusts (A.D.H.), the Glaucoma Research Foundation (A.D.H.), and grants from the US National Institutes of Health (R01 EY022157-01 to A.D.H. and R01 NS32405 to W.G.R.). S.R. is funded by an Alice and Joseph Brooks fellowship and a F32 NS087708 training grant and E.D. is funded by a F31 AG041582 (NIA) training grant.",
year = "2015",
month = aug,
day = "26",
doi = "10.1038/nn.4081",
language = "English (US)",
volume = "18",
pages = "1334--1341",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "9",
}