Cell separation using positive immunoselective techniques

Ross S. Basch, Joan W. Berman, Ellen Lakow

Research output: Contribution to journalReview article

34 Scopus citations

Abstract

Positive immunoselection is the direct selection and recovery of cells which express a given specificity from among a heterogeneous group of contaminating cells. A variety of methods are available to effect such separations. The principles of affinity chromatography, using solid-phase matrices or cellular immunoadsorbents, are extensively used. Liquid-phase positive immunoselection can also be performed using either a fluorescence-activated cell sorter or by using ‘cellular engineering’ to protect a cell from an otherwise noxious environment. The enzyme catalase coupled to specific antibody has been used for this purpose and renders cells resistant to hydrogen peroxide. The various positive immunoselection techniques available are reviewed and evaluated in the following report.

Original languageEnglish (US)
Pages (from-to)269-280
Number of pages12
JournalJournal of Immunological Methods
Volume56
Issue number3
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

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Keywords

  • biotin-avidin
  • fluorescence-activated cell-sorting affinity chromatography
  • immunoadsorbents
  • panning
  • positive immunoselection
  • rosettes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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