Cell invasion and matricide during Photorhabdus luminescens transmission by Heterorhabditis bacteriophora nematodes

Todd A. Ciche, Kwi Suk Kim, Bettina Kaufmann-Daszczuk, Ken C.Q. Nguyen, David H. Hall

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Many animals and plants have symbiotic relationships with beneficial bacteria. Experimentally tractable models are necessary to understand the processes involved in the selective transmission of symbiotic bacteria. One such model is the transmission of the insect-pathogenic bacterial symbionts Photorhabdus spp. by Heterorhabditis bactenophora infective juvenile (IJ)-stage nematodes. By observing egg-laying behavior and IJ development, it was determined that IJs develop exclusively via intrauterine hatching and matricide (i.e., endotokia matricida). By transiently exposing nematodes to fluorescently labeled symbionts, it was determined that symbionts infect the maternal intestine as a biofilm and then invade and breach the rectal gland epithelium, becoming available to the IJ offspring developing in the pseudocoelom. Cell- and stage-specific infection occurs again in the pre-IJ pharyngeal intestinal valve cells, which helps symbionts to persist as IJs develop and move to a new host. Synchronous with nematode development are changes in symbiont and host behavior (e.g., adherence versus invasion). Thus, Photorhabdus symbionts are maternally transmitted by an elaborate infectious process involving multiple selective steps in order to achieve symbiont-specific transmission.

Original languageEnglish (US)
Pages (from-to)2275-2287
Number of pages13
JournalApplied and Environmental Microbiology
Volume74
Issue number8
DOIs
StatePublished - Apr 2008

Fingerprint

Photorhabdus
Photorhabdus luminescens
Heterorhabditis bacteriophora
cell invasion
symbiont
symbionts
nematode
Salt Gland
Nematoda
Bacteria
nematode larvae
Biofilms
Intestines
Ovum
Insects
Epithelium
Mothers
Infection
Heterorhabditis
bacterium

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Cell invasion and matricide during Photorhabdus luminescens transmission by Heterorhabditis bacteriophora nematodes. / Ciche, Todd A.; Kim, Kwi Suk; Kaufmann-Daszczuk, Bettina; Nguyen, Ken C.Q.; Hall, David H.

In: Applied and Environmental Microbiology, Vol. 74, No. 8, 04.2008, p. 2275-2287.

Research output: Contribution to journalArticle

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