TY - JOUR
T1 - CD8+ T cells in HIV disease exhibit cytokine receptor perturbation and poor T cell receptor activation but are responsive to γ-chain cytokine-driven proliferation
AU - Pahwa, Rajendra
AU - McCloskey, Thomas W.
AU - Aroniadis, Olga C.
AU - Strbo, Natasa
AU - Krishnan, Subramaniam
AU - Pahwa, Savita
N1 - Funding Information:
Received 22 August 2005; accepted 4 October 2005; electronically published 9 February 2006. Presented in part: 11th Conference on Retrovirology and OpportunisticInfections, 8–11 February 2004, San Francisco, California (abstract 911). Potential conflicts of interest: none reported. Financial support: Public Health Service (grants AI 48857 and HD 37345 to S.P.). Reprints or correspondence: Dr. Savita Pahwa, Dept. of Microbiology and Immunology, University of Miami Miller School of Medicine, PO Box 016960 (R-138), 1600 NW 16th Ave., Miami, FL 33101 (spahwa@med.miami.edu).
PY - 2006/3/15
Y1 - 2006/3/15
N2 - Background. Cytokines are important for inducing T cell maturation, proliferation, and survival. Despite the known dysregulation of cytokines in human immunodeficiency virus (HIV) infection, cytokine receptor expression is relatively unexplored. Methods. We examined maturation markers (naive, central memory, effector memory, and effector); the cytokine receptors interleukin (IL)-2Rβ, common γ (Cγ) chain, IL-7Rα, IL-I5Rα; and proliferative responses of T cells in a cohort of HIV-infected pediatric patients (median age, 14.82 years) receiving antiretroviral therapy, arbitrarily designated as immunologic responders (group I) and nonresponders (group II) on the basis of a CD4+ T cell count cutoff of 25%. Results. Patients had increased percentages of effector memory CD8+ T cells, in comparison with those in healthy control subjects, with reduced expression of IL-7Rα in the central memory and effector memory subsets and of the Cγ chain in all maturation subsets of CD8+ T cells. IL- 7Rα+CD8+ T cell percentages were directly correlated with CD4+ T cell percentages. In immunologic nonresponders, anti-CD3+ or HIV Gag antigen-induced CD8+ T cell proliferation was impaired, but proliferation in response to the homeostatic cytokines IL-2 and IL-15 was preserved. Conclusions. Cytokine receptor deficiencies may contribute to immune deficiency in HIV-infected patients, and γ-chain-utilizing cytokines may play an important role in vivo in maintaining the memory subsets of T cells in patients with CD4+ T cell deficiency.
AB - Background. Cytokines are important for inducing T cell maturation, proliferation, and survival. Despite the known dysregulation of cytokines in human immunodeficiency virus (HIV) infection, cytokine receptor expression is relatively unexplored. Methods. We examined maturation markers (naive, central memory, effector memory, and effector); the cytokine receptors interleukin (IL)-2Rβ, common γ (Cγ) chain, IL-7Rα, IL-I5Rα; and proliferative responses of T cells in a cohort of HIV-infected pediatric patients (median age, 14.82 years) receiving antiretroviral therapy, arbitrarily designated as immunologic responders (group I) and nonresponders (group II) on the basis of a CD4+ T cell count cutoff of 25%. Results. Patients had increased percentages of effector memory CD8+ T cells, in comparison with those in healthy control subjects, with reduced expression of IL-7Rα in the central memory and effector memory subsets and of the Cγ chain in all maturation subsets of CD8+ T cells. IL- 7Rα+CD8+ T cell percentages were directly correlated with CD4+ T cell percentages. In immunologic nonresponders, anti-CD3+ or HIV Gag antigen-induced CD8+ T cell proliferation was impaired, but proliferation in response to the homeostatic cytokines IL-2 and IL-15 was preserved. Conclusions. Cytokine receptor deficiencies may contribute to immune deficiency in HIV-infected patients, and γ-chain-utilizing cytokines may play an important role in vivo in maintaining the memory subsets of T cells in patients with CD4+ T cell deficiency.
UR - http://www.scopus.com/inward/record.url?scp=33644909297&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644909297&partnerID=8YFLogxK
U2 - 10.1086/500471
DO - 10.1086/500471
M3 - Article
C2 - 16479523
AN - SCOPUS:33644909297
SN - 0022-1899
VL - 193
SP - 879
EP - 887
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -