CD4-T-cell antigen receptor complexes on human leukemia T cells

R. S. Chuck, C. R. Cantor, D. B. Tse

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

CD4 and T-cell antigen receptor (TCR) comodulate from the surface of human and murine T cells following exposure to monoclonal anti-CD4 or anti-TCR. This comodulation may occur because expression of CD4 and TCR is regulated by similar transmembrane signals or because CD4 and TCR are physically associated. To study multimolecular assemblies on the plasma membrane, we developed a flow cytometric method for detecting singlet-singlet energy transfer between fluorescein isothiocyanate (FITC)- and tetramethyl-rhodamine isothiocyanate (TRITC)-conjugated monoclonal antibodies as sensitized TRITC emission on intact, single cells. Using this procedure, we detected CD4-TCR complexes on the surface of the transformed human leukemia T cells, HPB-ALL, in the absence of stimulation. More than one CD4 were found in association with one TCR. CD4-TCR complexes were not in rapid equilibrium with free CD4 and free TCR, and they were not induced by the dye-labeled anti-CD4 or anti-TCR.

Original languageEnglish (US)
Pages (from-to)5021-5025
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number13
DOIs
StatePublished - Aug 6 1990
Externally publishedYes

Keywords

  • energy transfer
  • flow cytometry
  • molecular assemblies
  • receptors

ASJC Scopus subject areas

  • General

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