C. elegans ciliated sensory neurons release extracellular vesicles that function in animal communication

Juan Wang, Malan Silva, Leonard A. Haas, Natalia S. Morsci, Ken C.Q. Nguyen, David H. Hall, Maureen M. Barr

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Summary Cells release extracellular vesicles (ECVs) that play important roles in intercellular communication and may mediate a broad range of physiological and pathological processes [1-4]. Many fundamental aspects of ECV biogenesis and signaling have yet to be determined, with ECV detection being a challenge and obstacle due to the small size (100 nm) of the ECVs. We developed an in vivo system to visualize the dynamic release of GFP-labeled ECVs. We show here that specific Caenorhabdidits elegans ciliated sensory neurons shed and release ECVs containing GFP-tagged polycystins LOV-1 and PKD-2. These ECVs are also abundant in the lumen surrounding the cilium. Electron tomography and genetic analysis indicate that ECV biogenesis occurs via budding from the plasma membrane at the ciliary base and not via fusion of multivesicular bodies. Intraflagellar transport and kinesin-3 KLP-6 are required for environmental release of PKD-2::GFP-containing ECVs. ECVs isolated from wild-type animals induce male tail-chasing behavior, while ECVs isolated from klp-6 animals and lacking PKD-2::GFP do not. We conclude that environmentally released ECVs play a role in animal communication and mating-related behaviors.

Original languageEnglish (US)
Pages (from-to)519-525
Number of pages7
JournalCurrent Biology
Volume24
Issue number5
DOIs
StatePublished - Mar 3 2014

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Biocommunications
Animal Communication
sensory neurons
Sensory Receptor Cells
animal communication
Neurons
Animals
TRPP Cation Channels
Kinesin
kinesin
cell communication
sheds
tomography
cilia
Cell membranes
Tomography
genetic techniques and protocols
tail
Fusion reactions
plasma membrane

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

C. elegans ciliated sensory neurons release extracellular vesicles that function in animal communication. / Wang, Juan; Silva, Malan; Haas, Leonard A.; Morsci, Natalia S.; Nguyen, Ken C.Q.; Hall, David H.; Barr, Maureen M.

In: Current Biology, Vol. 24, No. 5, 03.03.2014, p. 519-525.

Research output: Contribution to journalArticle

Wang, Juan ; Silva, Malan ; Haas, Leonard A. ; Morsci, Natalia S. ; Nguyen, Ken C.Q. ; Hall, David H. ; Barr, Maureen M. / C. elegans ciliated sensory neurons release extracellular vesicles that function in animal communication. In: Current Biology. 2014 ; Vol. 24, No. 5. pp. 519-525.
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