Bone morphogenetic proteins induce astroglial differentiation of oligodendroglial-astroglial progenitor cells

P. C. Mabie, M. F. Mehler, R. Marmur, A. Papavasiliou, Q. Song, J. A. Kessler

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

We have used bipotent postnatal cortical oligodendroglial-astroglial progenitor cells (O-2As) to examine the role of inductive signals in astroglial lineage commitment. O-2A progenitor cells undergo progressive oligodendroglial differentiation when cultured in serum-free medium, but differentiate into astrocytes in medium supplemented with FBS. We now report that the bone morphogenetic proteins (BMPs), a major subclass of the transforming growth factor β (TGFβ) superfamily, promote the selective, dose-dependent differentiation of O-2As into astrocytes with concurrent suppression of oligodendroglial differentiation. This astroglial-inductive action is not sanctioned by other members of the TGFβ superfamily. Astroglial differentiation requires only very brief initial exposure to the BMPs and is accompanied by increased cellular survival and accelerated exit from cell cycle. Dual-label immunofluorescence microscopy documents that O- 2A progenitor cells express a complement of BMP type I and type II receptor subunits required for signal transduction. Furthermore, expression of BMP2 in vivo reaches maximal levels during the period of gliogenesis. These results suggest that the BMPs act as potent inductive factors in postnatal glial lineage commitment that initiate a stable program of astroglial differentiation.

Original languageEnglish (US)
Pages (from-to)4112-4120
Number of pages9
JournalJournal of Neuroscience
Volume17
Issue number11
StatePublished - 1997

Fingerprint

Bone Morphogenetic Proteins
Stem Cells
Transforming Growth Factors
Astrocytes
Type II Bone Morphogenetic Protein Receptors
Serum-Free Culture Media
Fluorescence Microscopy
Neuroglia
Signal Transduction
Cell Cycle

Keywords

  • astrocyte
  • bone morphogenetic protein
  • central nervous system development
  • lineage commitment
  • oligodendrocyte- astrocyte type 2 progenitor
  • transforming growth factor β superfamily

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bone morphogenetic proteins induce astroglial differentiation of oligodendroglial-astroglial progenitor cells. / Mabie, P. C.; Mehler, M. F.; Marmur, R.; Papavasiliou, A.; Song, Q.; Kessler, J. A.

In: Journal of Neuroscience, Vol. 17, No. 11, 1997, p. 4112-4120.

Research output: Contribution to journalArticle

Mabie, P. C. ; Mehler, M. F. ; Marmur, R. ; Papavasiliou, A. ; Song, Q. ; Kessler, J. A. / Bone morphogenetic proteins induce astroglial differentiation of oligodendroglial-astroglial progenitor cells. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 11. pp. 4112-4120.
@article{7dd1d1c2475e4b1bb2f4da5a797db09f,
title = "Bone morphogenetic proteins induce astroglial differentiation of oligodendroglial-astroglial progenitor cells",
abstract = "We have used bipotent postnatal cortical oligodendroglial-astroglial progenitor cells (O-2As) to examine the role of inductive signals in astroglial lineage commitment. O-2A progenitor cells undergo progressive oligodendroglial differentiation when cultured in serum-free medium, but differentiate into astrocytes in medium supplemented with FBS. We now report that the bone morphogenetic proteins (BMPs), a major subclass of the transforming growth factor β (TGFβ) superfamily, promote the selective, dose-dependent differentiation of O-2As into astrocytes with concurrent suppression of oligodendroglial differentiation. This astroglial-inductive action is not sanctioned by other members of the TGFβ superfamily. Astroglial differentiation requires only very brief initial exposure to the BMPs and is accompanied by increased cellular survival and accelerated exit from cell cycle. Dual-label immunofluorescence microscopy documents that O- 2A progenitor cells express a complement of BMP type I and type II receptor subunits required for signal transduction. Furthermore, expression of BMP2 in vivo reaches maximal levels during the period of gliogenesis. These results suggest that the BMPs act as potent inductive factors in postnatal glial lineage commitment that initiate a stable program of astroglial differentiation.",
keywords = "astrocyte, bone morphogenetic protein, central nervous system development, lineage commitment, oligodendrocyte- astrocyte type 2 progenitor, transforming growth factor β superfamily",
author = "Mabie, {P. C.} and Mehler, {M. F.} and R. Marmur and A. Papavasiliou and Q. Song and Kessler, {J. A.}",
year = "1997",
language = "English (US)",
volume = "17",
pages = "4112--4120",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "11",

}

TY - JOUR

T1 - Bone morphogenetic proteins induce astroglial differentiation of oligodendroglial-astroglial progenitor cells

AU - Mabie, P. C.

AU - Mehler, M. F.

AU - Marmur, R.

AU - Papavasiliou, A.

AU - Song, Q.

AU - Kessler, J. A.

PY - 1997

Y1 - 1997

N2 - We have used bipotent postnatal cortical oligodendroglial-astroglial progenitor cells (O-2As) to examine the role of inductive signals in astroglial lineage commitment. O-2A progenitor cells undergo progressive oligodendroglial differentiation when cultured in serum-free medium, but differentiate into astrocytes in medium supplemented with FBS. We now report that the bone morphogenetic proteins (BMPs), a major subclass of the transforming growth factor β (TGFβ) superfamily, promote the selective, dose-dependent differentiation of O-2As into astrocytes with concurrent suppression of oligodendroglial differentiation. This astroglial-inductive action is not sanctioned by other members of the TGFβ superfamily. Astroglial differentiation requires only very brief initial exposure to the BMPs and is accompanied by increased cellular survival and accelerated exit from cell cycle. Dual-label immunofluorescence microscopy documents that O- 2A progenitor cells express a complement of BMP type I and type II receptor subunits required for signal transduction. Furthermore, expression of BMP2 in vivo reaches maximal levels during the period of gliogenesis. These results suggest that the BMPs act as potent inductive factors in postnatal glial lineage commitment that initiate a stable program of astroglial differentiation.

AB - We have used bipotent postnatal cortical oligodendroglial-astroglial progenitor cells (O-2As) to examine the role of inductive signals in astroglial lineage commitment. O-2A progenitor cells undergo progressive oligodendroglial differentiation when cultured in serum-free medium, but differentiate into astrocytes in medium supplemented with FBS. We now report that the bone morphogenetic proteins (BMPs), a major subclass of the transforming growth factor β (TGFβ) superfamily, promote the selective, dose-dependent differentiation of O-2As into astrocytes with concurrent suppression of oligodendroglial differentiation. This astroglial-inductive action is not sanctioned by other members of the TGFβ superfamily. Astroglial differentiation requires only very brief initial exposure to the BMPs and is accompanied by increased cellular survival and accelerated exit from cell cycle. Dual-label immunofluorescence microscopy documents that O- 2A progenitor cells express a complement of BMP type I and type II receptor subunits required for signal transduction. Furthermore, expression of BMP2 in vivo reaches maximal levels during the period of gliogenesis. These results suggest that the BMPs act as potent inductive factors in postnatal glial lineage commitment that initiate a stable program of astroglial differentiation.

KW - astrocyte

KW - bone morphogenetic protein

KW - central nervous system development

KW - lineage commitment

KW - oligodendrocyte- astrocyte type 2 progenitor

KW - transforming growth factor β superfamily

UR - http://www.scopus.com/inward/record.url?scp=0030916680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030916680&partnerID=8YFLogxK

M3 - Article

C2 - 9151728

AN - SCOPUS:0030916680

VL - 17

SP - 4112

EP - 4120

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 11

ER -