Bissulfosuccinimidyl esters of aliphatic dicarboxylic acids: A new class of ‘affinity directed’ β ; crosslinkers of HBA

B. N. Manjula, R. P. Roy, P. K. Smith, A. S. Acharya

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The potential of sulfosuccinimidyl ester of suberic acid as an intramolecular crosslinker of HbA, directed to the positive charge rich domain of the protein (DPG pocket) has been investigated. The suberate ester introduced crosslinks between the/3-chains and inhibited the dissociation of HbA into α β dimers. The facile crosslinking reaction seen with this suberate ester as compared to the absence of crosslinking with the diaspirin derivative of suberic acid suggests the strong ‘steering’ influence of the sulfosuccinimidyl moiety of the reagent to the targeted site. The application of this crosslinking approach in the preparation of Hb based blood substitutes is discussed.

Original languageEnglish (US)
Pages (from-to)747-752
Number of pages6
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Volume22
Issue number3
DOIs
StatePublished - Jan 1994

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Bissulfosuccinimidyl esters of aliphatic dicarboxylic acids: A new class of ‘affinity directed’ β ; crosslinkers of HBA'. Together they form a unique fingerprint.

  • Cite this