Bissulfosuccinimidyl esters of aliphatic dicarboxylic acids

A new class of 'affinity directed' ββ crosslinkers of HbA

B. N. Manjula, R. P. Roy, P. K. Smith, A. S. Acharya

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The potential of sulfosuccinimidyl ester of suberic acid as an intramolecular crosslinker of HbA, directed to the positive charge rich domain of the protein (DPG pocket) has been investigated. The suberate ester introduced crosslinks between the β-chains and inhibited the dissociation of HbA into αβ dimers. The facile crosslinking reaction seen with this suberate ester as compared to the absence of crosslinking with the diaspirin derivative of suberic acid suggests the strong 'steering' influence of the sulfosuccinimidyl moiety of the reagent to the targeted site. The application of this crosslinking approach in the preparation of Hb based blood substitutes is discussed.

Original languageEnglish (US)
Pages (from-to)747-752
Number of pages6
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume22
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Dicarboxylic Acids
Crosslinking
Esters
Fatty Acids
Acids
Blood substitutes
Blood Substitutes
Dimers
Derivatives
Proteins
suberic acid

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials
  • Hematology

Cite this

Bissulfosuccinimidyl esters of aliphatic dicarboxylic acids : A new class of 'affinity directed' ββ crosslinkers of HbA. / Manjula, B. N.; Roy, R. P.; Smith, P. K.; Acharya, A. S.

In: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, Vol. 22, No. 3, 1994, p. 747-752.

Research output: Contribution to journalArticle

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T2 - A new class of 'affinity directed' ββ crosslinkers of HbA

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