@article{6fc89784a066418f89e5e639575db4a5,
title = "Bispecific antibody affords complete post-exposure protection of mice from both Ebola (Zaire) and Sudan viruses",
abstract = "Filoviruses (Ebola and Marburg) cause severe hemorrhagic fever. There are five species of ebolavirus; among these, the Ebola (Zaire) and Sudan viruses (EBOV and SUDV, respectively) are highly pathogenic and have both caused recurring, large outbreaks. However, the EBOV and SUDV glycoprotein (GP) sequences are 45% divergent and thus antigenically distinct. Few antibodies with cross-neutralizing properties have been described to date. We used antibody engineering to develop novel bispecific antibodies (Bis-mAbs) that are cross-reactive toward base epitopes on GP from EBOV and SUDV. These Bis-mAbs exhibit potent neutralization against EBOV and SUDV GP pseudotyped viruses as well as authentic pathogens, and confer a high degree (in one case 100%) post-exposure protection of mice from both viruses. Our studies show that a single agent that targets the GP base epitopes is sufficient for protection in mice; such agents could be included in panfilovirus therapeutic antibody cocktails.",
author = "Frei, {Julia C.} and Nyakatura, {Elisabeth K.} and Zak, {Samantha E.} and Bakken, {Russell R.} and Kartik Chandran and Dye, {John M.} and Lai, {Jonathan R.}",
note = "Funding Information: This work was supported by NIH grant U19 AI109762 (Centers for Excellence in Translational Research) to K.C., J.M.D. and J.R.L. and by an Irma T. Hirschl/Monique Weill-Caulier Research Scientist Award to J.R.L. J.C.F. was supported by NIH Cellular and Molecular Biology and Genetics Training Program T32-GM007491 and E.K.N. by a DAAD (Deutscher Akademischer Austausch Dienst, German Academic Exchange Service) fellowship. J.M.D. kindly acknowledges JSTO-DTRA project CB3948 for support Opinions, conclusions, interpretations, and recommendations are those of the authors and are not necessarily endorsed by the US. Army. The mention of trade names or commercial products does not constitute endorsement or recommendation for use by the Department of the Army or the Department of Defense.",
year = "2016",
month = jan,
day = "13",
doi = "10.1038/srep19193",
language = "English (US)",
volume = "6",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}