Biological systems analysis at molecular and subcellular levels: chromosome positioning inside human nuclei

Hui Bin Sun, Jin Shen, Omar El-Mounayri, Hiroki Yokota

Research output: Contribution to journalArticle


A systems mechanical analysis of biological samples, particularly DNA molecules and synthetic nuclei was carried out. Using optical, fluorescence, and atomic force microscopy, individual molecular and subcellular images of biological samples were detected. The mechanical properties of DNA molecules with and without bound-proteins were studied by applying small forces induced by surface tension or viscous drag and stretched DNA molecules. DNA molecules, modeled as random coil in solution, were straightened and immobilized on flat surface, and a stress-strain relationship was analyzed. Preliminary result suggests that the DNA molecules with bound-proteins behave differently in solution from the DNA molecules alone. By monitoring a process of nucleus formation, a linkage of a shape and a size of nuclei to a state of DNA and a stress field in a nucleus suspension was observed.

Original languageEnglish (US)
Pages (from-to)402-403
Number of pages2
JournalCritical Reviews in Biomedical Engineering
Issue number5
Publication statusPublished - Dec 1 1998
Externally publishedYes


ASJC Scopus subject areas

  • Biomedical Engineering

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