TY - JOUR
T1 - Biologic characteristics of specific human papillomavirus types predicted from morphology of cervical lesions
AU - Kadish, Anna S.
AU - Hagan, Robert J.
AU - Ritter, Diane B.
AU - Goldberg, Gary L.
AU - Romney, Seymour L.
AU - Kanetsky, Peter A.
AU - Beiss, Barbara K.
AU - Burk, Robert D.
N1 - Funding Information:
From the Departments of Pathology, Obstetrics and Gynecology, Pediatrics, and Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY. Accepted for publication February 19, 1992. Supported in part by grants from the National Institutes of Health no. l-ROl-CA47630, 5-P30-CA13330 and training grant no. CA-09173 (D.B.R and B.K.B.), and a grant from the Brookdale Foundation. Dr Burk was the recipient of a Faculty Research Award from the American Cancer Society. i<e?rw ordrt human papillomavirus, cervical intraepithelial neoplasia, oncologic potential, Southern blot hybridization. Papanicolaou smear, Bethesda system, immunohistochemistry, Ll major capsid antigen. squamous intraepithelial lesion. Address correspondence and reprint requests to Anna S. &dish, MD, Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Ave. Bronx, NY 1046 1. Copyright 0 1992 by W.B. Saunders Company 0046-8177/92/2311-0012$5.00/O
PY - 1992/11
Y1 - 1992/11
N2 - Human papillomavirus (HPV) DNA was detected by Southern blot hybridization in cervicovaginal lavage samples from 199 of 329 (60.5%) women attending a municipal hospital colposcopy clinic. Human papillomavirus was identified in 195 of 264 (73.9%) patients with a squamous intraepithelial lesion or cancer on biopsy or Papanicolaou smear (Bethesda system) compared with 11 of 65 (16.9%) without squamous intraepithelial lesion (P < .0001). The most common HPV type identified was HPV 16 (20.6% of positive samples), and 36.7% of isolates contained uncharacterized HPVs. Of women with cervical intraepithelial neoplasia (CIN) grade III or cancer, 23.4% were infected with HPV 16 compared with less than 4% with any other single HPV type. Based on biopsy diagnosis in patients infected with specific HPV types, HPVs 6 and 11 had low oncogenic potential; HPVs 18, 31, 35, and 45 had intermediate oncogenic potential; and HPVs 16 and 33 had high oncogenic potential. Hyperchromatic, unusually enlarged nuclei ("meganuclei"), and/or abnormal mitoses were found significantly more often in lesions infected with HPVs 16, 33, and 35 than in those infected with HPVs 6, 11, 18, 31, and 45, even in low-grade lesions, and may represent a histologic marker for HPVs with significant oncogenic potential. Human papillomavirus capsid protein was detected significantly less often by immunocytochemical staining in CIN I and CIN II lesions infected with HPVs 16 and 33 (8.3%) than in those infected with HPVs 6, 11, 18, and 31 (60%; P = .007), suggesting early abnormalities in cellular differentiation in lesions infected with highly oncogenic HPVs.
AB - Human papillomavirus (HPV) DNA was detected by Southern blot hybridization in cervicovaginal lavage samples from 199 of 329 (60.5%) women attending a municipal hospital colposcopy clinic. Human papillomavirus was identified in 195 of 264 (73.9%) patients with a squamous intraepithelial lesion or cancer on biopsy or Papanicolaou smear (Bethesda system) compared with 11 of 65 (16.9%) without squamous intraepithelial lesion (P < .0001). The most common HPV type identified was HPV 16 (20.6% of positive samples), and 36.7% of isolates contained uncharacterized HPVs. Of women with cervical intraepithelial neoplasia (CIN) grade III or cancer, 23.4% were infected with HPV 16 compared with less than 4% with any other single HPV type. Based on biopsy diagnosis in patients infected with specific HPV types, HPVs 6 and 11 had low oncogenic potential; HPVs 18, 31, 35, and 45 had intermediate oncogenic potential; and HPVs 16 and 33 had high oncogenic potential. Hyperchromatic, unusually enlarged nuclei ("meganuclei"), and/or abnormal mitoses were found significantly more often in lesions infected with HPVs 16, 33, and 35 than in those infected with HPVs 6, 11, 18, 31, and 45, even in low-grade lesions, and may represent a histologic marker for HPVs with significant oncogenic potential. Human papillomavirus capsid protein was detected significantly less often by immunocytochemical staining in CIN I and CIN II lesions infected with HPVs 16 and 33 (8.3%) than in those infected with HPVs 6, 11, 18, and 31 (60%; P = .007), suggesting early abnormalities in cellular differentiation in lesions infected with highly oncogenic HPVs.
KW - Bethesda system
KW - L1 major capsid antigen
KW - Papanicolaou smear
KW - Southern blot hybridization
KW - cervical intraepithelial neoplasia
KW - human papillomavirus
KW - immunohistochemistry
KW - oncologic potential
KW - squamous intraepithelial lesion
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U2 - 10.1016/0046-8177(92)90294-D
DO - 10.1016/0046-8177(92)90294-D
M3 - Article
C2 - 1330876
AN - SCOPUS:0026471914
SN - 0046-8177
VL - 23
SP - 1262
EP - 1269
JO - Human Pathology
JF - Human Pathology
IS - 11
ER -