Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro

Fang Xia Guan; Shan Shan Ma; Xin Yi Shi; Xun Ma; Lian Kai Chi; Shuo Liang; Yuan Bo Cui; Zhi Bin Wang; Ning Yao; Shao Kang Guan; Bo Yang

doi:10.1007/s11427-014-4610-9

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro

Fang Xia Guan, Shan Shan Ma, Xin Yi Shi, Xun Ma, Lian Kai Chi, Shuo Liang, Yuan Bo Cui, Zhi Bin Wang, Ning Yao, Shao Kang Guan, Bo Yang

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.

Original language	English (US)
Pages (from-to)	181-187
Number of pages	7
Journal	Science China Life Sciences
Volume	57
Issue number	2
DOIs	https://doi.org/10.1007/s11427-014-4610-9
State	Published - Feb 2014
Externally published	Yes

Keywords

Mg-Zn-Ca alloy
Wharton's jelly
biocompatibility
mesenchymal stem cells
nano-hydroxyapatite

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Environmental Science
General Agricultural and Biological Sciences

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11427-014-4610-9

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Guan, F. X., Ma, S. S., Shi, X. Y., Ma, X., Chi, L. K., Liang, S., Cui, Y. B., Wang, Z. B., Yao, N., Guan, S. K., & Yang, B. (2014). Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro. Science China Life Sciences, 57(2), 181-187. https://doi.org/10.1007/s11427-014-4610-9

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title = "Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro",

abstract = "Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.",

keywords = "Mg-Zn-Ca alloy, Wharton's jelly, biocompatibility, mesenchymal stem cells, nano-hydroxyapatite",

author = "Guan, {Fang Xia} and Ma, {Shan Shan} and Shi, {Xin Yi} and Xun Ma and Chi, {Lian Kai} and Shuo Liang and Cui, {Yuan Bo} and Wang, {Zhi Bin} and Ning Yao and Guan, {Shao Kang} and Bo Yang",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (81071008, 81171177, and 30870634), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA01030300), the Program for New Century Excellent Talents in University (NCET-06-0611), the Excellent Youth Foundation of Henan Scientific Committee (114100510005), the Young Excellent Teachers in University Funded Projects of Henan Province, and Bureau of Science and Technology Development Project from Henan Province (122102310203).",

year = "2014",

month = feb,

doi = "10.1007/s11427-014-4610-9",

language = "English (US)",

volume = "57",

pages = "181--187",

journal = "Science China Life Sciences",

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T1 - Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro

AU - Guan, Fang Xia

AU - Ma, Shan Shan

AU - Shi, Xin Yi

AU - Ma, Xun

AU - Chi, Lian Kai

AU - Liang, Shuo

AU - Cui, Yuan Bo

AU - Wang, Zhi Bin

AU - Yao, Ning

AU - Guan, Shao Kang

AU - Yang, Bo

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (81071008, 81171177, and 30870634), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA01030300), the Program for New Century Excellent Talents in University (NCET-06-0611), the Excellent Youth Foundation of Henan Scientific Committee (114100510005), the Young Excellent Teachers in University Funded Projects of Henan Province, and Bureau of Science and Technology Development Project from Henan Province (122102310203).

PY - 2014/2

Y1 - 2014/2

N2 - Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.

AB - Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.

KW - Mg-Zn-Ca alloy

KW - Wharton's jelly

KW - biocompatibility

KW - mesenchymal stem cells

KW - nano-hydroxyapatite

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Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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