Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro

Fangxia Guan, ShanShan S. Ma, XinYi Y. Shi, Xun Ma, LianKai K. Chi, Shuo Liang, YuanBo B. Cui, ZhiBin B. Wang, Ning Yao, ShaoKang K. Guan, Bo Yang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.

Original languageEnglish (US)
Pages (from-to)181-187
Number of pages7
JournalScience China Life Sciences
Volume57
Issue number2
DOIs
StatePublished - Feb 2014
Externally publishedYes

Fingerprint

Wharton Jelly
biocompatibility
umbilical cord
hydroxyapatite
jellies
Umbilical Cord
composite materials
Durapatite
Stem cells
seeding
Mesenchymal Stromal Cells
Biocompatibility
Scaffolds
stem cells
coating
tissue engineering
stem
engineering
Tissue Engineering
Tissue engineering

Keywords

  • biocompatibility
  • mesenchymal stem cells
  • Mg-Zn-Ca alloy
  • nano-hydroxyapatite
  • Wharton's jelly

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)

Cite this

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro. / Guan, Fangxia; Ma, ShanShan S.; Shi, XinYi Y.; Ma, Xun; Chi, LianKai K.; Liang, Shuo; Cui, YuanBo B.; Wang, ZhiBin B.; Yao, Ning; Guan, ShaoKang K.; Yang, Bo.

In: Science China Life Sciences, Vol. 57, No. 2, 02.2014, p. 181-187.

Research output: Contribution to journalArticle

Guan, Fangxia ; Ma, ShanShan S. ; Shi, XinYi Y. ; Ma, Xun ; Chi, LianKai K. ; Liang, Shuo ; Cui, YuanBo B. ; Wang, ZhiBin B. ; Yao, Ning ; Guan, ShaoKang K. ; Yang, Bo. / Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro. In: Science China Life Sciences. 2014 ; Vol. 57, No. 2. pp. 181-187.
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