TY - JOUR
T1 - Biochemical similarity among serologically distinct flagellins of Campylobacter jejuni
AU - Nachamkin, Irving
AU - Angeletti, Ruth Hogue
AU - Prystowsky, Michael B.
N1 - Funding Information:
We thank Xiao-He Yang for excellent technical assistance. Supported in part by BRSG SO7-RR-05415-26 from the National Institutes of Health.
PY - 1988/6/15
Y1 - 1988/6/15
N2 - We describe a simplified method for obtaining highly purified flagellin, suitable for biochemical analysis using HPLC-gel permeation. Amino acid composition and N-terminal sequence analyses were performed on flagellins from serologically distinct isolates. The amino acid composition of flagellin from 10 strains was very similar. The N-terminal amino acid sequence is highly conserved. Significant sequence homology was found with flagellin of Bacillus subtilis.
AB - We describe a simplified method for obtaining highly purified flagellin, suitable for biochemical analysis using HPLC-gel permeation. Amino acid composition and N-terminal sequence analyses were performed on flagellins from serologically distinct isolates. The amino acid composition of flagellin from 10 strains was very similar. The N-terminal amino acid sequence is highly conserved. Significant sequence homology was found with flagellin of Bacillus subtilis.
KW - (Amino acid sequence)
KW - (HPLC-gel permeation)
KW - Campylobacter jejuni
KW - Flagellin
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U2 - 10.1111/j.1574-6968.1988.tb02987.x
DO - 10.1111/j.1574-6968.1988.tb02987.x
M3 - Article
AN - SCOPUS:0023924942
SN - 0378-1097
VL - 51
SP - 149
EP - 152
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2-3
ER -