TY - JOUR
T1 - Berry intake changes hepatic gene expression and DNA methylation patterns associated with high-fat diet
AU - Heyman-Lindén, Lovisa
AU - Seki, Yoshinori
AU - Storm, Petter
AU - Jones, Helena A.
AU - Charron, Maureen J.
AU - Berger, Karin
AU - Holm, Cecilia
N1 - Funding Information:
Grant information: Financial support was provided through the Lund University Antidiabetic Food Centre , which is a VINNOVA VINN Excellence Center, the Swedish Research Council (project no. 112 84 to CH), the Swedish Diabetes Association , Crafoord Foundation , Royal Physiographic Society in Lund, A. Påhlsson Foundation and American Diabetes Association ( 1-13-CE-06 to MJC).
Funding Information:
The authors would like thank Sara Larsson and Maria Lindahl for excellent technical assistance and SCIBLU Genomics at Lund University. We are also grateful to Olga G?ransson for generously providing antibodies and fruitful discussions about AMPK. Ulrika Axling has given valuable advice and support throughout the study, and Florentina Negoita kindly performed a critical experiment. Furthermore, Lyda Williams and everyone in the laboratory of MJC, AECOM, provided valuable support and knowledge in the field of DNA methylation. Lastly, the authors are grateful to the AECOM epigenomics facility and Fabien Delahaye for HELP tag library and data analysis.
Publisher Copyright:
© 2015 The Authors.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - The liver is a critical organ for regulation of energy homeostasis and fatty liver disease is closely associated with obesity and insulin resistance. We have previously found that lingonberries, blackcurrants and bilberries prevent, whereas açai berries exacerbate, the development of hepatic steatosis and obesity in the high-fat (HF)-fed C57BL/6J mouse model. In this follow-up study, we investigated the mechanisms behind these effects. Genome-wide hepatic gene expression profiling indicates that the protective effects of lingonberries and bilberries are accounted for by several-fold downregulation of genes involved in acute-phase and inflammatory pathways (e.g. Saa1, Cxcl1, Lcn2). In contrast, açai-fed mice exhibit marked upregulation of genes associated with steatosis (e.g. Cfd, Cidea, Crat) and lipid and cholesterol biosynthesis, which is in line with the exacerbation of HF-induced hepatic steatosis in these mice. In silico transcription factor analysis together with immunoblot analysis identified NF-κB, STAT3 and mTOR as upstream regulators involved in mediating the observed transcriptional effects. To gain further insight into mechanisms involved in the gene expression changes, the HELP-tagging assay was used to identify differentially methylated CpG sites. Compared to the HF control group, lingonberries induced genome-wide hypermethylation and specific hypermethylation of Ncor2, encoding the corepressor NCoR/SMRT implicated in the regulation of pathways of metabolic homeostasis and inflammation. We conclude that the beneficial metabolic effects of lingonberries and bilberries are associated with downregulation of inflammatory pathways, whereas for blackcurrants, exerting similar metabolic effects, different mechanisms of action appear to dominate. NF-κB, STAT3 and mTOR are potential targets of the health-promoting effects of berries.
AB - The liver is a critical organ for regulation of energy homeostasis and fatty liver disease is closely associated with obesity and insulin resistance. We have previously found that lingonberries, blackcurrants and bilberries prevent, whereas açai berries exacerbate, the development of hepatic steatosis and obesity in the high-fat (HF)-fed C57BL/6J mouse model. In this follow-up study, we investigated the mechanisms behind these effects. Genome-wide hepatic gene expression profiling indicates that the protective effects of lingonberries and bilberries are accounted for by several-fold downregulation of genes involved in acute-phase and inflammatory pathways (e.g. Saa1, Cxcl1, Lcn2). In contrast, açai-fed mice exhibit marked upregulation of genes associated with steatosis (e.g. Cfd, Cidea, Crat) and lipid and cholesterol biosynthesis, which is in line with the exacerbation of HF-induced hepatic steatosis in these mice. In silico transcription factor analysis together with immunoblot analysis identified NF-κB, STAT3 and mTOR as upstream regulators involved in mediating the observed transcriptional effects. To gain further insight into mechanisms involved in the gene expression changes, the HELP-tagging assay was used to identify differentially methylated CpG sites. Compared to the HF control group, lingonberries induced genome-wide hypermethylation and specific hypermethylation of Ncor2, encoding the corepressor NCoR/SMRT implicated in the regulation of pathways of metabolic homeostasis and inflammation. We conclude that the beneficial metabolic effects of lingonberries and bilberries are associated with downregulation of inflammatory pathways, whereas for blackcurrants, exerting similar metabolic effects, different mechanisms of action appear to dominate. NF-κB, STAT3 and mTOR are potential targets of the health-promoting effects of berries.
KW - Berries
KW - Gene expression
KW - High-fat diet
KW - Inflammation
KW - Liver steatosis
KW - Methylation
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U2 - 10.1016/j.jnutbio.2015.08.022
DO - 10.1016/j.jnutbio.2015.08.022
M3 - Article
C2 - 26423886
AN - SCOPUS:84952864920
VL - 27
SP - 79
EP - 95
JO - Nutrition Reports International
JF - Nutrition Reports International
SN - 0955-2863
ER -