Basic fibroblast growth factor among children with diarrhea-associated hemolytic uremic syndrome

Patricio Ray, David Acheson, Ramona Chitrakar, Avital Cnaan, Kathleen Gibbs, Gladys H. Hirschman, Erica Christen, Howard Trachtman, Julie Ingelfinger, Josephine Briggs, John Kusek, Daniel Cattran, Mitchell B. Cohen, Katherine Freeman, Thomas Greene, Solomon Moshe, Seth Schulman, James Springate, Frederick Kaskel, Dilys WhyteRobert Weiss, Charles McKay, Lewis Reisman, Eduardo Perelstein, Manju Chandra, Jose Salcedo, Lynne Weiss, William Varade, Douglas Ford, James Chan, Irene Restaino, Shashi Nagaraj, Victoria Norwood, John Foreman, Michael Moritz, John Mahan, Marva Moxey-Mims, Barry Warshaw, Verna Yiu, Andrew Brem, Sharon Bartosh, Sharon Andreoli, Lawrence Milner, Jens Goebel, Dianne Muchant, Coral Hanevold

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Diarrhea-associated hemolytic uremic syndrome (D+HUS) is characterized by endothelial injury and activation of inflammatory cytokines. Basic fibroblast growth factor (bFGF) is an angiogenic peptide released in response to vascular damage. The plasma concentrations and urinary excretion of bFGF during the course of D+HUS were determined, in comparison with the levels of various inflammatory cytokines, and changes were correlated with clinical and laboratory features of the disease. Serial plasma and urine samples were collected from 31 children with D+HUS, during the acute (days 1 to 7 of hospitalization) and recovery (through day 60 after discharge from the hospital) phases of the disease. The patients were enrolled in the multicenter trial of SYNSORB Pk (SYNSORB Biotech, Calgary, Alberta, Canada) treatment for D+HUS. bFGF, interleukin-1α (IL-1α), IL-8, and tumor necrosis factor-α levels were determined with enzyme-linked immunosorbent assays. bFGF was detected in urine and plasma samples more frequently than were IL-1α, IL-8, and tumor necrosis factor-α. There was an acute increase in urinary bFGF excretion, which returned to normal during convalescence. Urinary excretion of bFGF during the acute phase was higher among patients who required dialysis, compared with those who did not (48.9 ± 15.0 and 28.9 ± 9.0 pg/ml, respectively; P < 0.05). Plasma bFGF concentrations were persistently elevated throughout the period of hospitalization and the follow-up period among patients with D+HUS. Urinary excretion and plasma levels of bFGF were comparable for the SYNSORB Pk-treated (n = 19) and placebo-treated (n = 12) groups. Measurements of urinary and plasma concentrations of bFGF among patients with D+HUS may be useful indices for assessment of the severity of acute renal disease and the timing and adequacy of the systemic angiogenic process during early convalescence.

Original languageEnglish (US)
Pages (from-to)699-707
Number of pages9
JournalJournal of the American Society of Nephrology
Volume13
Issue number3
StatePublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Nephrology

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