B Cells Regulate Neutrophilia during Mycobacterium tuberculosis Infection and BCG Vaccination by Modulating the Interleukin-17 Response

Lee Kozakiewicz, Yong Chen, Jiayong Xu, Yanhua Wang, Kyri Dunussi-Joannopoulos, Qinglin Ou, Joanne L. Flynn, Steven A. Porcelli, William R. Jacobs, John Chan

Research output: Contribution to journalArticle

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Abstract

We have previously demonstrated that B cells can shape the immune response to Mycobacterium tuberculosis, including the level of neutrophil infiltration and granulomatous inflammation at the site of infection. The present study examined the mechanisms by which B cells regulate the host neutrophilic response upon exposure to mycobacteria and how neutrophilia may influence vaccine efficacy. To address these questions, a murine aerosol infection tuberculosis (TB) model and an intradermal (ID) ear BCG immunization mouse model, involving both the μMT strain and B cell-depleted C57BL/6 mice, were used. IL (interleukin)-17 neutralization and neutrophil depletion experiments using these systems provide evidence that B cells can regulate neutrophilia by modulating the IL-17 response during M. tuberculosis infection and BCG immunization. Exuberant neutrophilia at the site of immunization in B cell-deficient mice adversely affects dendritic cell (DC) migration to the draining lymph nodes and attenuates the development of the vaccine-induced Th1 response. The results suggest that B cells are required for the development of optimal protective anti-TB immunity upon BCG vaccination by regulating the IL-17/neutrophilic response. Administration of sera derived from M. tuberculosis-infected C57BL/6 wild-type mice reverses the lung neutrophilia phenotype in tuberculous μMT mice. Together, these observations provide insight into the mechanisms by which B cells and humoral immunity modulate vaccine-induced Th1 response and regulate neutrophila during M. tuberculosis infection and BCG immunization.

Original languageEnglish (US)
Article numbere1003472
JournalPLoS Pathogens
Volume9
Issue number7
DOIs
StatePublished - Jul 2013

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Mycobacterium Infections
Interleukin-17
Mycobacterium bovis
Mycobacterium tuberculosis
Vaccination
B-Lymphocytes
Immunization
Vaccines
Tuberculosis
Neutrophil Infiltration
Cell Shape
Mycobacterium
Humoral Immunity
Infection
Aerosols
Inbred C57BL Mouse
Dendritic Cells
Cell Movement
Ear
Immunity

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

B Cells Regulate Neutrophilia during Mycobacterium tuberculosis Infection and BCG Vaccination by Modulating the Interleukin-17 Response. / Kozakiewicz, Lee; Chen, Yong; Xu, Jiayong; Wang, Yanhua; Dunussi-Joannopoulos, Kyri; Ou, Qinglin; Flynn, Joanne L.; Porcelli, Steven A.; Jacobs, William R.; Chan, John.

In: PLoS Pathogens, Vol. 9, No. 7, e1003472, 07.2013.

Research output: Contribution to journalArticle

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