ATP released from astrocytes mediates glial calcium waves

Peter B. Guthrie, Joshua Knappenberger, Menahem Segal, Michael V. L. Bennett, Andrew C. Charles, Stanley B. Kater

Research output: Contribution to journalArticle

588 Citations (Scopus)

Abstract

Calcium waves represent a widespread form of intercellular communication. Although they have been thought for a long time to require gap junctions, we recently demonstrated that mouse cortical astrocytes use an extracellular messenger for calcium wave propagation. The present experiments identify ATP as a major extracellular messenger in this system. Medium collected from astrocyte cultures during (but not before) calcium wave stimulation contains ATP. The excitatory effects of medium samples and of ATP are blocked by purinergic receptor antagonists and by pretreatment with apyrase; these same purinergic receptor antagonists block propagation of electrically evoked calcium waves. ATP, applied at the concentration measured in medium samples, evokes responses that are qualitatively and quantitatively similar to those evoked by those medium samples. These data implicate ATP as an important transmitter between CNS astrocytes.

Original languageEnglish (US)
Pages (from-to)520-528
Number of pages9
JournalJournal of Neuroscience
Volume19
Issue number2
StatePublished - Jan 15 1999

Fingerprint

Calcium Signaling
Neuroglia
Astrocytes
Adenosine Triphosphate
Purinergic Antagonists
Apyrase
Gap Junctions

Keywords

  • Apyrase
  • Astrocytes
  • ATP
  • Calcium waves
  • Electrical stimulation
  • Extracellular signal
  • Glia
  • Purinergic
  • Suramin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Guthrie, P. B., Knappenberger, J., Segal, M., Bennett, M. V. L., Charles, A. C., & Kater, S. B. (1999). ATP released from astrocytes mediates glial calcium waves. Journal of Neuroscience, 19(2), 520-528.

ATP released from astrocytes mediates glial calcium waves. / Guthrie, Peter B.; Knappenberger, Joshua; Segal, Menahem; Bennett, Michael V. L.; Charles, Andrew C.; Kater, Stanley B.

In: Journal of Neuroscience, Vol. 19, No. 2, 15.01.1999, p. 520-528.

Research output: Contribution to journalArticle

Guthrie, PB, Knappenberger, J, Segal, M, Bennett, MVL, Charles, AC & Kater, SB 1999, 'ATP released from astrocytes mediates glial calcium waves', Journal of Neuroscience, vol. 19, no. 2, pp. 520-528.
Guthrie PB, Knappenberger J, Segal M, Bennett MVL, Charles AC, Kater SB. ATP released from astrocytes mediates glial calcium waves. Journal of Neuroscience. 1999 Jan 15;19(2):520-528.
Guthrie, Peter B. ; Knappenberger, Joshua ; Segal, Menahem ; Bennett, Michael V. L. ; Charles, Andrew C. ; Kater, Stanley B. / ATP released from astrocytes mediates glial calcium waves. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 2. pp. 520-528.
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